Sensitization to mechanical stimulation by inflammatory mediators and by mild burn in canine visceral nociceptors in vitro

Journal of Neurophysiology
Hisashi Koda, K Mizumura

Abstract

Hyperalgesia to mechanical stimulation and heat is commonly observed in inflamed conditions. Although sensitization to heat is well documented and its mechanism has also been well studied, it remains unclear whether and how nociceptors are sensitized to mechanical stimulation. Therefore we conducted in vitro investigation of which inflammatory mediators (bradykinin, histamine, prostaglandin E2, and protons) sensitize nociceptors to suprathreshold mechanical stimulation and at what concentrations. In addition, we studied the effects of possible second messengers for these mediators downstream of the receptors and also the effects of mild burn. Single polymodal receptor activities were recorded in canine testis-spermatic nerve preparations excised from deeply anesthetized dogs. Mechanical stimulation was applied to the identified receptive field for 10 s with a servo-controlled mechanical stimulator. Bradykinin at 0.001 microM induced neither excitation nor facilitation of the mechanical response; however, it facilitated the mechanical response at 0.01 microM and higher, levels at which significant excitation was also induced by bradykinin alone. Histamine excited the nociceptor and sensitized it to mechanical stimulation at 10 m...Continue Reading

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Citations

May 8, 2007·The Journal of Pharmacology and Experimental Therapeutics·Xi-Chun ZhangDan Levy
Nov 10, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Liang-Wu Fu, John C Longhurst
Oct 31, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stephen D Skaper, Laura Facci
Sep 17, 2013·Immunology·Stephen D SkaperPietro Giusti
Feb 7, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·L BuenoN J Talley
Aug 25, 2007·Anesthesia and Analgesia·Michael A ThackerStephen B McMahon
Apr 28, 2009·Experimental Brain Research·Kazue MizumuraYasuko Kozaki
Dec 25, 2009·Pain·Jun Ho JangTimothy J Brennan
Apr 21, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephen D SkaperLaura Facci
Dec 4, 2019·American Journal of Physiology. Renal Physiology·Luke GrundyStuart M Brierley
Jun 1, 2013·Psychosomatic Medicine·Toyohiro HamaguchiShin Fukudo
Oct 16, 2015·The Journal of Physiological Sciences : JPS·Kazue Mizumura, Toru Taguchi
May 6, 2016·Frontiers in Immunology·Andrea I LoewendorfMike Harrington

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