PMID: 9181234Jun 1, 1997Paper

Sensitizing T-lymphocytes for adoptive immunotherapy by vaccination with wild-type or cytokine gene-transduced melanoma

Annals of Surgical Oncology
K A LipshyH D Bear

Abstract

For the relatively nonimmunogenic B16-F10 murine melanoma, it has been found that genetically engineered expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) but not interleukin (IL)-2, IL-4, or interferon-gamma (IFN-gamma) resulted in a vaccine that could induce resistance to rechallenge. Because T cells from lymph nodes draining the sites of some progressive tumors can mediate tumor regression after in vitro activation, it seemed possible that even apparently nonimmunogenic melanoma cells might induce similar preeffector cells in the vaccine-draining lymph nodes (DLNs). C57BL/6 mice were vaccinated with B16-F10 cells that were either unmodified or genetically modified to produce IL-2, IL-4, GM-CSF, or IFN-gamma. DLNs were harvested 10 days after vaccination for adoptive immunotherapy (AIT). The DLN cells were activated with bryostatin 1 and ionomycin (B/I), expanded for 10 days in culture, and transferred to mice with 3-day pulmonary metastases. Pulmonary nodules were counted 14 days after AIT. Adoptive transfer of expanded DLN lymphocytes sensitized by inoculation of WT B16-F10, or IL-4, GM-CSF, or IFN-gamma expressing cells significantly reduced pulmonary metastases. Despite the spontaneous regression of ...Continue Reading

References

Oct 15, 1990·International Journal of Cancer. Journal International Du Cancer·R KellerP H van der Meide
Oct 1, 1990·The Journal of Experimental Medicine·B GansbacherE Gilboa
Jan 1, 1988·Annual Review of Immunology·M Herlyn, H Koprowski
Aug 29, 1970·Nature·G R PettitH B Wood
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·W SchmidtM L Birnstiel
Aug 1, 1994·Journal of Immunotherapy with Emphasis on Tumor Immunology : Official Journal of the Society for Biological Therapy·J C KraussS Shu
Apr 1, 1993·Journal of Immunotherapy with Emphasis on Tumor Immunology : Official Journal of the Society for Biological Therapy·J D GeigerA E Chang

❮ Previous
Next ❯

Citations

Jun 19, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sheinei J SaleemDaniel H Conrad
Apr 24, 2015·International Journal of Molecular Sciences·Christine Kathryn ZoonHarry D Bear
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H M HuB A Fox
Jun 25, 2002·Expert Opinion on Biological Therapy·Bela Bodey
Mar 24, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·D M Pardoll

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.