Sensor manufacturer, temperature, and cyanobacteria morphology affect phycocyanin fluorescence measurements

Environmental Science and Pollution Research International
Caroline M HodgesDavid P Hamilton

Abstract

Sensors to measure phycocyanin fluorescence in situ are becoming widely used as they may provide useful proxies for cyanobacterial biomass. In this study, we assessed five phycocyanin sensors from three different manufacturers. A combination of culture-based experiments and a 30-sample field study was used to examine the effect of temperature and cyanobacteria morphology on phycocyanin fluorescence. Phycocyanin fluorescence increased with decrease in temperature, although this varied with manufacturer and cyanobacterial density. Phycocyanin fluorescence and cyanobacterial biovolume were strongly correlated (R 2 > 0.83, P < 0.05) for single-celled and filamentous species. The relationship was generally weak for a colonial strain of Microcystis aeruginosa. The colonial culture was divided into different colony size classes and phycocyanin measured before and after manual disaggregation. No differences were measured, and the observation that fluorescence spiked when large colonial aggregates drifted past the light source suggests that sample heterogeneity, rather than lack of light penetration into the colonies, was the main cause of the poor relationship. Analysis of field samples showed a strong relationship between in situ phyc...Continue Reading

References

Aug 1, 1973·The Journal of Cell Biology·A Bennett, L Bogorad
Mar 2, 2005·Toxicology and Applied Pharmacology·Geoffrey A CoddJames S Metcalf
Feb 5, 2008·Journal of Environmental Monitoring : JEM·Luc BrientMyriam Bormans
Nov 26, 2010·Journal of Environmental Monitoring : JEM·Christian BastienIsabelle Laurion
Dec 14, 2011·Journal of Environmental Monitoring : JEM·A ZamyadiS Dorner
Feb 12, 2013·Environmental Science and Pollution Research International·Kaishan SongYing Sun

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