Sensors for biosensors: a novel tandem monitoring in a droplet towards efficient screening of robust design and optimal operating conditions

The Analyst
Daria SemenovaKrist V Gernaey

Abstract

Understanding the biorecognition and transduction mechanisms is a key aspect in the development of robust sensing technologies. Therefore, the design of tools and analytical approaches that could allow gaining a deeper insight into the bio- and electrochemical processes would significantly accelerate the progress in the field of biosensors. Herein, we present a novel effective strategy for biosensor design screening based on tandem monitoring of individual system parameters in a droplet. The developed tandem approach couples the simultaneous chronoamperometric characterization of biosensors in the presence of an analyte (glucose) together with dissolved oxygen monitoring using a luminescence-based optical oxygen microsensor. Remarkably, an optical sensor was applied for the first time to analyse the amperometric biosensor response and kinetics. Two types of multi-layer glucose biosensors (first generation) were chosen as a case study and were evaluated at various operating conditions using multi-analytical techniques. Moreover, specific protocols were developed for the detection of oxygen conversion rates, iron and membrane elution inside the multi-layer glucose biosensor system. The presented tandem monitoring approach allows ...Continue Reading

References

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Methods Mentioned

BETA
biosensor
biosensors
scanning
X-ray
scanning electron microscopy

Software Mentioned

Pyro Oxygen Logger
MultiTrace
MultiEmStat

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