Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses

Molecular and Cellular Probes
Baback GharizadehNader Pourmand

Abstract

Despite the various technologies in place for genotyping human papillomaviruses (HPV), clinical use and clinical research demand a method that is fast, more reliable and cost-effective. The technology described here represents a breakthrough development in that direction. By combining the method of multiple sequencing primers with DNA sequencing, we have developed a rapid assay for genotyping HPV that relies on the identification of a single, type-specific 'sentinel' base. As described here, the prototype assay has been developed to recognize the 12 most high-risk HPV types (HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59) and is capable of recognizing and simultaneously genotyping multiple HPV co-infections. By providing sequence information on multiple HPV infections, this method eliminates the need for labor- and cost-intensive PCR cloning. These proof-of-concept studies establish the assay to be accurate, reliable, rapid, flexible, and cost-effective, providing evidence of the feasibility this technique for use in clinical settings.

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Citations

Apr 1, 2008·Journal of Biosciences·Cheryl M TravassoChandan Kumar-Sinha
Jul 14, 2012·The American Journal of Surgical Pathology·Anne M MillsChristina S Kong
Apr 17, 2009·Journal of Clinical Microbiology·Jae-jin ChoiHeekyung Park
Nov 16, 2006·BMC Bioinformatics·Sreedevi ThiyagarajanNader Pourmand
Dec 22, 2006·PloS One·Nader PourmandRonald W Davis
Feb 22, 2007·PloS One·Michael S AkhrasNader Pourmand
Oct 12, 2013·PloS One·Michael S AkhrasNader Pourmand
Nov 27, 2007·Journal of Microbiological Methods·Rogerio C NovaisYvonne R Thorstenson
Jul 7, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Björn StranderAgneta Andersson-Ellström
May 12, 2012·The Journal of Molecular Diagnostics : JMD·Myungsok OhSungwhan An

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