Sentinel node lymphocytes: tumour reactive lymphocytes identified intraoperatively for the use in immunotherapy of colon cancer.

British Journal of Cancer
P MaritsO Winqvist

Abstract

The sentinel node is the first lymph node to receive lymphatic drainage from a tumour and is usually the first site of metastases. Today, the sentinel node is used for tumour staging. Here, we focus on its immunological role and investigate lymphocytic function in sentinel nodes, identified intraoperatively by peritumoural dye injection, from 15 patients with colon cancer. Tumour infiltrating lymphocytes, sentinel and nonsentinel lymph node cells and peripheral blood leukocytes were studied by flow cytometry, proliferation assays and interferon-gamma secretion after activation with autologous tumour homogenate. Whereas tumour-infiltrating lymphocytes were nonresponsive in the proliferation assays, lymphocytes from sentinel nodes proliferated dose dependently and secreted interferon-gamma upon stimulation with tumour homogenate. The responses were of varying magnitude and tended to be weaker in metastatic sentinel nodes. Sentinel node lymphocytes represents an enriched source of tumour reactive lymphocytes, and may be useful in future trials of adoptive immunotherapy.

References

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Citations

Feb 27, 2008·Annals of Surgical Oncology·Kjell DahlMagnus Thörn
Feb 2, 2010·Annals of Surgical Oncology·Mona KarlssonOla Winqvist
Oct 3, 2007·Journal of Internal Medicine·G Klein
Sep 7, 2016·British Journal of Cancer·Ciputra Adijaya HartanaOla Winqvist
May 27, 2010·Scandinavian Journal of Immunology·M KarlssonM Eberhardson
Oct 7, 2015·Current Colorectal Cancer Reports·Timothy J Zumwalt, Ajay Goel
Jun 21, 2019·Journal of Experimental & Clinical Cancer Research : CR·Stephan KrugerMichael von Bergwelt-Baildon
Jan 5, 2012·Scandinavian Journal of Gastroenterology·Eva EllebaekPer Thor Straten
Aug 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Peter C J JansonOla Winqvist
May 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·A Ali ZirakzadehOla Winqvist
Apr 2, 2008·Journal of Immunological Methods·Mona KarlssonOla Winqvist

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Methods Mentioned

BETA
biopsies
flow cytometry
FCS
ELISA
dissection
surgical resection

Software Mentioned

Cellquest

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