Separate actions of different colony stimulating factors from human placental conditioned medium on human hemopoietic progenitor cell survival and proliferation

Journal of Cellular Physiology
G MorstynD Metcalf

Abstract

More than 20% of human granulocyte-macrophage and eosinophil colony-forming cells survived in agar culture for up to 4 days without the addition of exogenous colony stimulating factors (human placental-conditioned medium, HPCM). Survival was reduced slightly but not significantly, by the removal of adherent cell populations. Significant survival occurred even when only 100 cells enriched for colony-forming cells (CFCs) were cultured per dish. When individual colonies, initiated by stimulation with HPCM for 5 days, were transferred to dishes without HPCM, subsequent proliferation was significantly reduced compared with control cultures containing HPCM. Using the fluorescence-activated cell sorter and the fluoresceinated lectin from Lotus tetragonolobus, two populations of marrow cells were obtained, one enriched for day 7 and the other for day 14 colony-forming cells. Two colony-stimulating factors fractionated from HPLCM (CSF beta and CSF alpha) have been shown previously to stimulate the day 7 and day 14 colony-forming cell populations, respectively. Developing clones from cultures initiated with CSF beta died between the fifth and tenth day of culture after transfer to dishes with CSF alpha or CSF beta or to dishes with no st...Continue Reading

References

Oct 1, 1975·Journal of Cellular Physiology·F L BakerP R Galbraith
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Jun 1, 1974·Journal of Cellular Physiology·H S Lin, C C Stewart
Jan 1, 1981·Scandinavian Journal of Haematology·G MorstynJ W Fabre

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Citations

Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·T M Dexter
Sep 15, 2004·Tissue Engineering·Gerald G WulfLorenz Trümper
Apr 8, 1999·Leukemia & Lymphoma·H G DrexlerH Quentmeier
Jan 1, 1989·Cancer Investigation·G MorstynJ E Layton
Jan 1, 1986·International Journal of Cell Cloning·K Rhyner, R Taetle
Jun 1, 1987·Australian and New Zealand Journal of Medicine·W P SheridanG Morstyn
Dec 1, 1981·Australian and New Zealand Journal of Medicine·G MorstynT Hurley

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