Separate analysis of human papillomavirus E6 and E7 messenger RNAs to predict cervical neoplasia progression

PloS One
Shuling LiuToyomi Satoh

Abstract

A few studies previously suggested that human papillomavirus (HPV) E6 messenger RNA (mRNA) may exist uniformly in all grades of cervical intraepithelial neoplasia (CIN), whereas the detection rate of E7 mRNA may increase with disease progression from low-grade CIN to invasive carcinoma. The aim of this study was to clarify the different roles of E6 and E7 mRNAs in cervical carcinogenesis. The presence of each E6 and E7 mRNA was analyzed in 171 patients with pathologically-diagnosed CIN or cervical carcinoma. We utilized a RT-PCR assay based on consensus primers which could detect E6 mRNA (full-length E6/E7 transcript) and E7 mRNAs (spliced E6*/E7 transcripts) separately for various HPV types. E7 mRNAs were detected in 6% of CIN1, 12% of CIN2, 24% of CIN3, and 54% of cervical carcinoma. The presence of E7 mRNAs was significantly associated with progression from low-grade CIN to invasive carcinoma in contrast with E6 mRNA or high-risk HPV (HR-HPV) DNA (p = 0.00011, 0.80 and 0.54). The presence of both E6 and E7 mRNAs was significantly associated with HPV16/18 DNA but not with HR-HPV DNA (p = 0.0079 and 0.21), while the presence of E6 mRNA was significantly associated with HR-HPV DNA but not with HPV16/18 DNA (p = 0.036 and 0.089)...Continue Reading

References

Apr 16, 2004·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Arnold-Jan KruseJan P A Baak
Sep 22, 2011·Cytopathology : Official Journal of the British Society for Clinical Cytology·M Waldstrom, D Ornskov
Mar 15, 2012·The Journal of Obstetrics and Gynaecology Research·Takashi TakedaUNKNOWN Japan Association of Obstetricians and Gynecologists
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Aug 24, 2016·The Nurse Practitioner

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Methods Mentioned

BETA
genotyping
PCR
transgenic

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