Separate analysis of twin-arginine translocation (Tat)-specific membrane binding and translocation in Escherichia coli.

The Journal of Biological Chemistry
Meriem AlamiMatthias Müller

Abstract

The twin-arginine translocation (Tat) pathway exports those precursor proteins to the periplasmic space of bacteria that harbor a twin-arginine (RR) consensus motif in their signal sequences. We have reproduced translocation of several Tat substrates into inside-out plasma membrane vesicles from Escherichia coli. Translocation proceeding at an efficiency of up to 20% occurs specifically via the Tat pathway as indicated by (i) its requirement for elevated levels of the TatABC proteins in the membrane vesicles, (ii) competition by an intact twin-arginine signal peptide, and (iii) susceptibility toward dissipation of the transmembrane H(+) gradient. The latter treatment, while blocking translocation, still allows for functional membrane association of Tat precursors. This is shown by the finding that translocation of isolated membrane-bound Tat precursor is restored upon re-energization of the vesicles.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M J Casadaban, S N Cohen
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M Müller, G Blobel
Jan 29, 2000·Molecular Microbiology·B C BerksT Palmer
Oct 29, 2000·Progress in Nucleic Acid Research and Molecular Biology·M MüllerU Schäfer
Apr 20, 2001·Molecular Microbiology·I J OresnikR J Turner
May 2, 2001·Nature Reviews. Molecular Cell Biology·C Robinson, A Bolhuis
May 15, 2001·The EMBO Journal·T L Yahr, W T Wickner

❮ Previous
Next ❯

Citations

Apr 27, 2004·Trends in Biochemical Sciences·Tracy PalmerBen C Berks
Jun 18, 2003·Biochemical and Biophysical Research Communications·Nathalie PradelLong-Fei Wu
Oct 9, 2002·Journal of Molecular Biology·Erik de LeeuwBen C Berks
Dec 20, 2012·Nature Communications·Julia FröbelMatthias Müller
Mar 13, 2003·European Journal of Biochemistry·Thomas BrüserFevzi Daldal
Nov 2, 2011·The Journal of Biological Chemistry·Julia FröbelMatthias Müller
Oct 7, 2008·The Journal of Biological Chemistry·Sascha PanahandehMatthias Müller
Oct 12, 2010·Molecular Biology of the Cell·Carlo MaurerMatthias Müller
Mar 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Julia FröbelMatthias Müller
Dec 8, 2005·Applied and Environmental Microbiology·Jae-Young KimMatthew P DeLisa
Dec 4, 2012·Journal of Bacteriology·Meghan E FeltcherMiriam Braunstein
Jan 2, 2004·Journal of Bacteriology·Matthew P DeLisaGeorge Georgiou
Jun 8, 2006·Annual Review of Microbiology·Philip A LeeGeorge Georgiou
Jun 29, 2011·Journal of Molecular Microbiology and Biotechnology·Barbara MaldonadoTracy Palmer
Oct 10, 2012·The Journal of Cell Biology·Rebecca KellerTracy Palmer
Oct 3, 2007·The Journal of Cell Biology·Umesh K Bageshwar, Siegfried M Musser
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·J Amaranath GovindanGary Ruvkun
Mar 17, 2010·Biochemical and Biophysical Research Communications·Ken-ichi NishiyamaHajime Tokuda
Mar 21, 2007·Research in Microbiology·Emanuel Maltempi de SouzaM Geoffrey Yates
Mar 8, 2005·Research in Microbiology·Matthias Müller
Mar 2, 2005·Biochemical and Biophysical Research Communications·Christopher A McDevittBen C Berks
Mar 30, 2005·Gene·Matthias Paschke, Wolfgang Höhne
Mar 4, 2014·Biochimica Et Biophysica Acta·Roshani PatelColin Robinson
Dec 1, 2011·Biomolecular Concepts·Bo Hou, Thomas Brüser
Nov 18, 2004·Biochimica Et Biophysica Acta·Colin Robinson, Albert Bolhuis
Mar 1, 2003·Microbiological Research·Thomas Brüser, Carsten Sanders

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.