PMID: 7082864Jul 1, 1982Paper

Separate detection of glucose-6-phosphate dehydrogenase from 6-phosphogluconate dehydrogenase by DEAE-paper chromatography

Blut
H MisumiM Kosaka

Abstract

Red blood cell lysates were applied to DEAE cellulose paper for detection of glucose-6-phosphate dehydrogenase. Glucose-6-phosphate dehydrogenase (G-6-PD) was separated from 6-phosphogluconate dehydrogenase (6-PGD) by DEAE-cellulose paper-chromatography and their activities were detected on the chromatogram by applying the reaction mixture. The fluorescence of NADPH formed by G-6-PD was not interfered with by 6-PGD activity. This is a sensitive procedure which detects slight loss of G-6-PD in red blood cells and could be applied to the detection of G-6-PD deficiency.

Related Concepts

Chromatography, DEAE-Cellulose
Paper Chromatography (Procedure)
Erythrocytes
Glucosephosphate Dehydrogenase
Deficiency of glucose-6-phosphate Dehydrogenase
Phosphogluconate Dehydrogenase

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