PMID: 9178617Jun 1, 1997Paper

Separate determination of the electrical properties of the tonoplast and the plasmalemma of the giant-celled alga Valonia utricularis: vacuolar perfusion of turgescent cells with nystatin and other agents

The Journal of Membrane Biology
J WangU Zimmermann

Abstract

In the giant-celled marine algae Valonia utricularis the turgor-sensing mechanism of the plasmalemma and the role of the tonoplast in turgor regulation is unknown because of the lack of solid data about the individual electrical properties of the plasmalemma and the vacuolar membrane. For this reason, a vacuolar perfusion technique was developed that allowed controlled manipulation of the vacuolar sap under turgescent conditions (up to about 0.3 MPa). Charge-pulse relaxation studies on vacuolarly perfused cells at different turgor pressure values showed that the area-specific resistance of the total membrane barrier (tonoplast and plasmalemma) exhibited a similar dependence on turgor pressure as reported in the literature for nonperfused cells: the resistance assumed a minimum value at the physiological turgor pressure of about 0.1 MPa. The agreement of the data suggested that the perfusion process did not alter the transport properties of the membrane barrier. Addition of 16 microM of the H+-carrier FCCP (carbonylcyanide p-trifluoromethoxyphenyhydrazone) to the perfusion solution resulted in a drop of the total membrane potential from +4 mV to -22 mV and in an increase of the area-specific membrane resistance from 6.8 x 10(-2)...Continue Reading

Citations

Apr 6, 2019·Frontiers in Human Neuroscience·Jinbiao LiuHonghai Liu

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