Separating extracellular vesicles and lipoproteins via acoustofluidics

Lab on a Chip
Mengxi WuTony Jun Huang

Abstract

Extracellular vesicles (EVs) and lipoproteins are abundant and co-exist in blood. Both have been proven to be valuable as diagnostic biomarkers and for therapeutics. However, EVs and lipoproteins are both on the submicron scale and overlap in size distributions. Conventional methods to separate EVs and lipoproteins are inefficient and time-consuming. Here we present an acoustofluidic-based separation technique that is based on the acoustic property differences of EVs and lipoproteins. By using the acoustofluidic technology, EVs and subgroups of lipoproteins are separated in a label-free, contact-free, and continuous manner. With its ability for simple, rapid, efficient, continuous-flow isolation, our acoustofluidic technology could be a valuable tool for health monitoring, disease diagnosis, and personalized medicine.

References

Jan 1, 1977·Annual Review of Biochemistry·J L Goldstein, M S Brown
Dec 1, 1991·The Journal of Clinical Investigation·J L Witztum, D Steinberg
May 29, 1998·Circulation·P W WilsonW B Kannel
Jul 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lawrence RajendranKai Simons
Jun 16, 2007·Analytical Chemistry·Filip PeterssonThomas Laurell
Jul 11, 2007·Current Opinion in Lipidology·Stephen J Nicholls, Steven E Nissen
Jan 16, 2009·Trends in Cell Biology·Emanuele CocucciJacopo Meldolesi
Jun 6, 2009·Nature Reviews. Immunology·Clotilde ThéryElodie Segura
Jan 1, 2011·Journal of Lipids·Angeliki ChroniHelen Karlsson
Apr 16, 2013·Nature Reviews. Drug Discovery·Samir EL AndaloussiMatthew J A Wood
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Vedashree RamakrishnaiahLuc J W van der Laan
Aug 1, 2013·Lab on a Chip·Xiaoyun DingTony Jun Huang
Jan 24, 2014·Journal of Extracellular Vesicles·Yuana YuanaSusanne Osanto
Apr 8, 2014·Trends in Molecular Medicine·Pieter VaderMatthew J A Wood
Jul 16, 2014·Journal of Extracellular Vesicles·Yuana YuanaRienk Nieuwland
Aug 12, 2014·Journal of Translational Medicine·Suchismita SarkerCarlos Salomon
Oct 4, 2014·Journal of Extracellular Vesicles·Anita N BöingRienk Nieuwland
Feb 13, 2015·ACS Nano·Kyungheon LeeHakho Lee
Mar 15, 2015·Methods : a Companion to Methods in Enzymology·Douglas D Taylor, Sahil Shah
Mar 31, 2015·Journal of Extracellular Vesicles·Joanne Louise WeltonAled Clayton
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Peng LiTony Jun Huang
Dec 25, 2015·Lab on a Chip·Zhangming MaoTony Jun Huang
Mar 10, 2016·Bioscience·MikoŁaj P ZaborowskiCharles P Lai
Sep 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Mengxi WuTony Jun Huang
Dec 30, 2017·International Journal of Molecular Sciences·Jiong-Wei WangDominique P V de Kleijn

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Citations

Nov 14, 2019·Lab on a Chip·Tianlong ZhangMing Li
Dec 13, 2019·Small·Alinaghi SalariScott S H Tsai
Feb 15, 2020·Biology of Reproduction·Eleanore V O'NeilThomas E Spencer
Jun 13, 2020·Annual Review of Analytical Chemistry·Peiran ZhangTony Jun Huang
Feb 6, 2020·Seminars in Immunopathology·Laura S PetersonBrice Gaudilliere
Jan 29, 2021·Lab on a Chip·Minji KimJ Mark Meacham
Jan 21, 2021·Frontiers in Veterinary Science·Bahram TalebjediMarcia De Almeida Monteiro Melo Ferraz
Mar 14, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Janine StamJustina C Wolters
Jan 16, 2021·ACS Biomaterials Science & Engineering·Michael M BinkleyJ Mark Meacham
Apr 30, 2021·Cell Biology International·Ghadi N AlzhraniNehal Elsherbiny
Aug 4, 2021·The Journal of the Acoustical Society of America·Mehrshad Rezadoost DezfuliMajid Ghassemi
Oct 9, 2019·Analytical Chemistry·Elnaz MirtaheriBilal El-Zahab
Dec 1, 2020·ACS Nano·Yuliang XieTony Jun Huang
Sep 4, 2021·Lab on a Chip·Shu-Kun ZhaoYi Yang
Nov 2, 2021·ACS Nano·Sara Hassanpour TamrinArindom Sen

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Methods Mentioned

BETA
ELISA
electrophoresis

Software Mentioned

Image J
COMSOL Multiphysics®

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