Separating stereoisomers of di-, tri-, and tetrapeptides using capillary electrophoresis with contactless conductivity detection

Journal of Separation Science
Xiao Yang GongPeter C Hauser

Abstract

The separation and detection of small oligopeptides in CE with contactless conductivity detection were demonstrated. A strongly acidic separation buffer (0.5 M acetic acid) was employed in order to render the species cationic. Separation of the stereoisomers was achieved in typically 10-15 min by using either dimethyl-beta-CD (DM-beta-CD), (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18C(6)H(4)), a combination of the two substances, or of histidine, as buffer additives. Calibration curves were determined for isomers of Gly-Asp and H-Pro-Asp-NH(2), in the range of 0.05-0.5 mM and 0.1-1 mM, respectively, and were found to be linear. LODs were determined to be in the order of 1.0 microM. The determination of isomeric impurities down to about 1% was found possible. Species showing good separation could also be successfully determined on an electrophoretic lab-on-chip device, with analysis times of a few minutes.

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Citations

Jul 22, 2014·Journal of Separation Science·Imran AliAlexander Chudinov
Dec 2, 2009·Electrophoresis·Václav Kasicka
Dec 30, 2009·Electrophoresis·Laura Sánchez-HernándezAntonio Luis Crego
Aug 5, 2009·Electrophoresis·Stefan NaglDetlev Belder
Mar 30, 2011·Analytical Chemistry·Stefan NaglDetlev Belder
May 6, 2015·Analytical Chemistry·Sebastian ThurmannDetlev Belder
Mar 26, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Worapan PormsilaPeter C Hauser

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