Separation and analysis of dynamic Stokes shift with multiple fluorescence environments: coumarin 153 in bovine beta-lactoglobulin A

The Journal of Physical Chemistry. B
Jeremy PronchikDavid S Talaga

Abstract

We use time-dependent fluorescence Stokes shift (TDFSS) information to study the fluctuation rates of the lipocalin, beta-lactoglobulin A in the vicinity of an encapsulated coumarin 153 molecule. The system has three unique dielectric environments in which the fluorophore binds. We develop a method to decompose the static and dynamic contributions to the spectral heterogeneity. This method is applied to temperature-dependent steady-state fluorescence spectra providing us with site-specific information about thermodynamic transitions in beta-lactoglobulin. We confirm previously reported transitions and discuss the presence of an unreported transition of the central calyx at 18 degrees C. Our method also resolves the contributions to the TDFSS from the coumarin 153 centrally located in the calyx of beta-lactoglobulin despite overlapping signals from solvent exposed dyes. Our experiments show dynamics ranging from 3-12,00 ps. The analysis shows a decrease in the encapsulated dye's heterogeneity during the relaxation, which is taken as evidence of the breakdown of linear response.

References

Oct 1, 1988·The Biochemical Journal·N K Kella, J E Kinsella
Jan 1, 1988·European Biophysics Journal : EBJ·A P Demchenko
Dec 1, 1993·Protein Science : a Publication of the Protein Society·P D Thomas, K A Dill
Jun 1, 1997·Journal of Dairy Science·Q WangH E Swaisgood
Dec 29, 1998·The Journal of Biological Chemistry·S Y WuL Sawyer
Apr 14, 1999·Nature Structural Biology·R M Ionescu, C R Matthews
Dec 14, 1999·Protein Science : a Publication of the Protein Society·K KuwataY Goto
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·W A EatonJ Hofrichter
Dec 6, 2000·Protein Science : a Publication of the Protein Society·M ColliniG Baldini
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·D ZhongA H Zewail
Apr 28, 2001·Biophysical Journal·J T Vivian, P R Callis
Sep 22, 2001·Biochimica Et Biophysica Acta·T Lefèvre, M Subirade
Oct 19, 2001·European Journal of Biochemistry·D FessasF Bonomi
Jan 30, 2002·Luminescence : the Journal of Biological and Chemical Luminescence·Alexander P Demchenko
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Samir Kumar PalAhmed H Zewail
May 25, 2002·Critical Reviews in Biochemistry and Molecular Biology·Chung-Jung TsaiRuth Nussinov
Jun 12, 2004·Photochemistry and Photobiology·P K ChowdhuryJ W Petrich
Jul 21, 2004·Journal of Dairy Science·G KontopidisL Sawyer
May 8, 2007·Biophysical Journal·Troy C Messina, David S Talaga
Jun 27, 2007·The Journal of Physical Chemistry. B·Paul AbbyadSteven G Boxer
Sep 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kazumasa Sakurai, Yuji Goto
Jan 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kalyan DasEddy Arnold

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