PMID: 9416468Jan 7, 1998Paper

Separation and characterization of cholesteryl oxo- and hydroxy-linoleate isolated from human atherosclerotic plaque

Free Radical Research
C SuarnaR Stocker

Abstract

In previous work we demonstrated that up to 30% of cholesteryl linoleate in homogenates of advanced human plaque samples is present in oxidized forms. Here we show that the material from plaque hexane extracts which co-elutes with cholesteryl hydroxy-linoleate on reversed phase HPLC (Anal Biochem 1993;213:79), is composed of several isomers of cholesteryl hydroxy- and cholesteryl oxo-octadecadienoate. Enzymatic hydrolysis and measurement of liberated cholesterol and disappearance of the esters revealed that almost all of the material consisted of unoxidized cholesterol esterified to oxidized derivatives of octadecadienoate. Semi-preparative reversed-phase HPLC was used to obtain sufficient quantities of this co-eluting material to undertake normal phase HPLC separation of these components. The nature of such separated and isolated compounds was identified, by co-chromatography with authentic standards, UV spectroscopy and chemical ionization and electron impact mass spectrometry, as cholesteryl hydroxy- and cholesteryl oxo-octadecadienoate. These oxidized fatty acids have been observed previously in plaque, in agreement with our new unambiguous demonstration of their presence as cholesteryl esters. The application of the method...Continue Reading

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Citations

Mar 4, 2009·Molecular Biotechnology·Arnis KuksisHeikki Kallio
Jun 8, 2001·Free Radical Biology & Medicine·M J ThomasL L Rudel
Sep 18, 1998·Current Opinion in Lipidology·S Tsimikas, P D Reaven
Mar 3, 2009·Molecular Aspects of Medicine·Giuseppe PoliGabriella Leonarduzzi
Aug 9, 2005·The American Journal of Medicine·Joshua F BakerH Ralph Schumacher
Jun 15, 2007·Free Radical Biology & Medicine·Angelo Azzi
Sep 24, 2004·Physiological Reviews·Roland Stocker, John F Keaney
Apr 17, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·S R ThomasR Stocker
May 25, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Carr, B Frei
May 25, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J M UpstonR Stocker

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