PMID: 6973357Jun 15, 1981Paper

Separation and characterization of hexokinase I subtypes from human erythrocytes

Biochimica Et Biophysica Acta
G RijksenG E Staal

Abstract

Hexokinase (ATP: D-hexose 6-phosphotransferase, EC 2.7.1.1) type 1 from human erythrocytes exists in four electrophoretical distinct forms, termed Ia, Ib, Ic and Id in order of their increasing anodal electrophoretic mobility at pH 8.8. We were able to separate type Ia, Ib and Icd on phosphocellulose by using a discontinuous gradient elution. The three chromatographically distinct forms do not differ in their affinity constants for the substrates glucose and MgATP2-. In addition the inhibition by glucose 1,6-diphosphate does not differ significantly for all forms. However, the regulation of these inhibitions by inorganic phosphate is much less for type Ia compared to the other subtypes (P = 0.001). Aging of the red cells is accompanied by a relative increase of the proportion of type Ic and Ia, which is the less regulated form of the enzyme. This shift in electrophoretic and regulatory properties is argued to be due to a post-translational modification of the primary enzyme.

References

Oct 1, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·G RijksenG E Staal
Feb 9, 1979·Biochimica Et Biophysica Acta·D T Vowles, J S Easterby
Sep 14, 1976·Biochimica Et Biophysica Acta·G Rijksen, G E Staal
Jan 26, 1976·Biochemical and Biophysical Research Communications·P L Felgner, J E Wilson
Oct 5, 1973·European Journal of Biochemistry·J S Easterby, M J O'Brien
Jun 1, 1971·The American Journal of Clinical Nutrition·J W AndersonR M Cohn
Oct 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·H M Katzen, R T Schimke
Sep 1, 1980·Biochimica Et Biophysica Acta·R J KraaijenhagenG E Staal

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Citations

Jun 27, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·R J KraaijenhagenG E Staal
Apr 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·D R Thorburn, P W Kuchel
Sep 1, 1985·British Journal of Haematology·G JansenG E Staal
Oct 17, 1986·Biochimica Et Biophysica Acta·A A Haritos, M A Rosemeyer
Jan 4, 1983·Biochimica Et Biophysica Acta·E D SprengersG E Staal
Mar 1, 1986·Archives of Biochemistry and Biophysics·M MagnaniG Fornaini
Jan 1, 1988·Archives of Biochemistry and Biophysics·M MagnaniG Fornaini
May 1, 1988·Archives of Biochemistry and Biophysics·H M Katzen, D D Soderman

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