Separation and characterization of mares' milk alpha(s1)-, beta-, kappa-caseins, gamma-casein-like, and proteose peptone component 5-like peptides

Journal of Dairy Science
A S EgitoJ L Gaillard

Abstract

The equine alpha(s1)- and beta-caseins (CN) were purified by chromatography on DEAE-cellulose and by reversed-phase HPLC. The alpha(s1)-, beta-, and kappa-CN were characterized either by monodimensional urea-PAGE or sodium dodecylsulfate (SDS)-PAGE or by bidimensional electrophoresis. Kappa-casein was characterized after electrophoresis by glycoprotein-specific staining. To identify alpha(s1)-CN without ambiguity, internal sequences were determined after trypsin or chymosin digestion of purified alpha(s1)-CN. These sequences, that could be estimated to correspond to 62% of the full protein, presented strong identities with regions of alpha(s1)-CN primary structures of other species. In particular, 51, 48, 43, and 40% identities were obtained with corresponding regions of sow, dromedary, cow, and human alpha(s1)-CN, respectively. On the other hand, trace amounts of equine gamma-CN-like and proteose peptone component 5-like peptides were found in the whole CN. They were identified by microsequencing and corresponded to beta-CN peptides generated by plasmin action on the whole CN. The equine alpha(s1), beta-, and kappa-CN were separated by bidimensional electrophoresis in numerous isoelectric variants with apparent isoelectric poi...Continue Reading

References

May 1, 1976·Journal of Dairy Science·C Kotts, R Jenness
May 1, 1991·The Journal of Dairy Research·L K Rasmussen, T E Petersen
Dec 1, 1973·Analytical Biochemistry·R A Kapitany, E J Zebrowski
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Apr 1, 1982·The Biochemical Journal·S VisserR J Mullin
Apr 6, 2001·The Journal of Dairy Research·B S IamettiF Bonomi

❮ Previous
Next ❯

Citations

Mar 1, 2012·The Journal of Dairy Research·Katharina HinzAlan L Kelly
Jan 7, 2015·Journal of Biomolecular Structure & Dynamics·Zainab Moosavi-MovahediAli A Moosavi-Movahedi
Jan 17, 2015·Animal : an International Journal of Animal Bioscience·Y QinS Zou
May 6, 2013·Foods·Paolo Polidori, Silvia Vincenzetti
Apr 25, 2016·Journal of Dairy Science·J BrinkmannJ Tetens
Jun 21, 2018·The Journal of Dairy Research·Michele FacciaAngela G D'Alessandro
Feb 22, 2012·Animal Science Journal = Nihon Chikusan Gakkaihō·Ewa PeckaKatarzyna Czyż
Aug 30, 2008·Molecular Nutrition & Food Research·Cristina BarelloMaria Gabriella Giuffrida
May 21, 2010·Rapid Communications in Mass Spectrometry : RCM·Aurélie MatéosLaurent Miclo
Oct 26, 2005·Veterinary Research Communications·S VincenzettiA Vita
Dec 28, 2019·Foods·Michele FacciaAngela Gabriella D'Alessandro
Nov 5, 2020·International Journal of Molecular Sciences·Agnieszka RodzikBogusław Buszewski
Feb 19, 2013·Journal of Agricultural and Food Chemistry·Therese Uniacke-LoweDaniel M Mulvihill
Jul 10, 2013·Journal of Agricultural and Food Chemistry·Oun Ki ChangJun-Sang Ham
Mar 14, 2008·International Journal of Biological Macromolecules·Abolfazl BarzegarAli Akbar Moosavi-Movahedi
Apr 18, 2009·Colloids and Surfaces. B, Biointerfaces·Jalil BadraghiAli Akbar Moosavi-Movahedi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.