Separation and characterization of silybin, isosilybin, silydianin and silychristin in milk thistle extract by liquid chromatography-electrospray tandem mass spectrometry

Journal of Chromatography. a
James I LeeJeffrey S Barrett

Abstract

A selective and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed for the characterization of silymarin in commercially available milk thistle extract. In this study, six main active constituents, including silydianin, silychristin, diastereomers of silybin (silybin A and B) and diastereomers of isosilybin (isosilybin A and B) in silymarin, were completely separated on a YMC ODS-AQ HPLC column using a gradient mobile phase system comprised of ammonium acetate and methanol/water/formic acid. Identification and characterization of the major constituents were based not only on the product ion scan, which provided unique fragmentation information of a selected molecular ion, but also on the specific fragmentation of multiple reaction monitoring (MRM) data, which confirmed the retention times of LC chromatographic peaks. The method was applied in the analysis of human plasma samples in the presence of silymarin and appeared to be suitable for the pharmacokinetic studies in which the discrimination of silymarin constituents is essential.

References

Feb 19, 1998·The American Journal of Gastroenterology·K FloraK Benner
Aug 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V SimánekL Cvak
Jul 14, 2001·Journal of Pharmaceutical and Biomedical Analysis·T DingZ Sun
Aug 25, 2001·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·K Wellington, B Jarvis
Dec 12, 2001·Drugs·R SallerR Brignoli
Oct 9, 2002·Journal of Pharmaceutical and Biomedical Analysis·Anna Rita BiliaFranco Francesco Vincieri
Apr 11, 2003·Journal of Chromatography. a·F KvasnickaJ Krátká
Feb 4, 2005·Phytochemical Analysis : PCA·Sunny N WallaceEdgar C Clausen
Sep 20, 2005·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Vladimír Kren, Daniela Walterová
Nov 1, 2005·Journal of Viral Hepatitis·K E MayerS S Lee

❮ Previous
Next ❯

Citations

May 7, 2008·The American Journal of Chinese Medicine·Kuo-Lung KuHui-Fen Liao
Jun 3, 2011·World Journal of Gastroenterology : WJG·Carmela Loguercio, Davide Festi
Nov 29, 2015·Chemico-biological Interactions·Sohair R FahmySara M Elaskalany
Jan 11, 2011·Journal of Pharmaceutical and Biomedical Analysis·S P LiB Yang
May 18, 2013·Rapid Communications in Mass Spectrometry : RCM·Lajos NagySándor Kéki
Oct 18, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Luca CalaniDaniele Del Rio
Mar 31, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Abel Felipe FreitagRoberto Kenji Nakamura Cuman
Dec 30, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mustafa CengizAdnan Ayhancı
Jun 5, 2014·Journal of Chromatographic Science·Dorota Wianowska, Mariusz Wiśniewski
Jun 21, 2016·Journal of Pharmaceutical and Biomedical Analysis·Dezső CsuporJudit Hohmann
Dec 7, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei LiChangxiao Liu
Jun 6, 2007·Integrative Cancer Therapies·David J KrollNicholas H Oberlies
Nov 5, 2014·Food & Function·Carla PereiraIsabel C F R Ferreira
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Mar 30, 2017·Frontiers in Pharmacology·Saúl Redondo-BlancoFelipe Lombó
Jul 15, 2015·Archives of Pharmacal Research·Xiaodan Zhou, Zhipeng Chen
Apr 15, 2020·Allergologia et immunopathologia·Entezar Mehrabi NasabSeyyed Shamsadin Athari
Sep 3, 2020·Journal of Ethnopharmacology·Ilias MarmouziMourad Kharbach
Apr 1, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Abdoh TalebDing Qilong
Oct 14, 2020·Current Drug Targets·Shailaja Dombe, Pramodkumar Shirote

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.