Separation and characterization of two related giardiaviruses in the parasitic protozoan Giardia lamblia

Virology
J H TaiS J Ong

Abstract

Giardiavirus encapsidates a 6.2-kb double-stranded (ds) RNA within a capsid that consists of a major 100-kDa capsid protein (p100) and a minor 190-kDa protein (p190). In this study, two nonhomologous 6.2-kb ds RNAs cohabiting in Giardia lamblia trophozoites were found to be separately encapsidated into two distinct virions, one (designated GLV[p100]) whose capsid consists of p100 and p190, and the other (designated GLV[p95]) whose capsid consists of a 95-kDa protein (p95) and a minor p190-equivalent protein. Both types of virions were enriched in the membranous fraction of a lysate from virus-infected G. lamblia cells. Separation of these virions was achieved by CsCl gradient centrifugation following osmotic rupture of the viral particles. By these treatments, the 6.2-kb ds RNA was removed from GLV[p100] whereas that in GLV[p95] remained unchanged, and the two 6.2-kb ds RNAs that had been purified by this protocol displayed differential hybridization properties to viral cDNA probes. Western blotting and peptide mapping experiments show that p100 and p95 were closely related proteins, but each had distinct amino acid sequences. Virus purification and pulse-chase experiments show that GLV[p100] was selectively secreted into the m...Continue Reading

Citations

Jul 4, 2001·Clinical Microbiology Reviews·R D Adam
Feb 25, 2009·Experimental Parasitology·Simone M Cacciò, Hein Sprong
Dec 22, 2007·International Journal for Parasitology·M C JenkinsR Fayer
Apr 25, 2017·European Journal of Dentistry·Ayesha HanifHaroon Rashid
Apr 30, 2008·Journal of Applied Animal Welfare Science : JAAWS·Nicole CottamGary J Patronek

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