Separation and confirmation of nine Enterobacteriaceae strains that carry the bla NDM-1 gene

Experimental and Therapeutic Medicine
Tian-Jiao LiY E Lu

Abstract

The aim of the present study was to confirm the existence of carbapenem-resistant Enterobacteriaceae carrying the bla NDM-1 gene in clinics in Hainan province, China. Collected clinical bacterial isolates that were Enterobacteriaceae strains suspected of producing carbapenemase were used as experimental strains. Drug resistance to imipenem, meropenem and other antibacterial agents was tested. Imipenem/imipenem inhibitor (IP/IPI) E-testing was conducted to identify the bacterial strains that produced metallo-β-lactamases. The bla NDM-1 drug resistance gene was amplified by polymerase chain reaction (PCR), and agarose gel electrophoresis (AGE) and sequencing were conducted to identify the products. The species of the strains carrying the bla NDM-1 gene were determined using a biochemical identification system. Through the IP/IPI E-test, 21 of the 30 collected Enterobacteriaceae strains were found to be positive, indicating that 70% of the strains produced metallo-β-lactamases. Following bla NDM-1 gene PCR amplification, AGE and sequencing tests confirmed that nine of the strains carried the bla NDM-1 drug resistance gene. The biochemical identification system indicated that four of the strains were Klebsiella pneumoniae, two were...Continue Reading

References

Jun 20, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xi ChengWenxiang Jia
Nov 26, 2010·Journal of the Chinese Medical Association : JCMA·Hua-Shin WuShou-Dong Lee
Oct 18, 2011·Emerging Infectious Diseases·Patrice NordmannLaurent Poirel

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Datasets Mentioned

BETA
FN396876.1
KC573881.1
KC573880.1
KC573878.1

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

DNAStar
ClustalW

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