Separation and determination of alpha-synuclein monomeric and oligomeric species using two electrophoretic approaches

Electrophoresis
Aurore NappMarianne Fillet

Abstract

Parkinson's disease (PD) is a frequent degenerative disorder that is diagnosed based on clinical symptoms. When the first symptoms appear, more than 70% of the dopaminergic cells are already lost. Therefore, it is of utmost importance to have reliable biomarkers to diagnose much earlier PD. In this context, alpha-synuclein (aSyn) is a protein of high interest because of its tendency to form oligomers and amyloid fibrils. The oligomeric forms seem to play a critical pathological role in PD. To date, most of studies aiming at detecting and quantifying aSyn oligomers were performed by immunoassays, mainly by ELISA using specific antibodies. In this study a capillary gel electrophoresis (CGE) coupled with fluorescence detection method was developed to detect and quantify the oligomeric forms of aSyn formed in vitro. All the results obtained were supported by SDS-PAGE analysis, a widely used and well-known technique but exhibiting a main drawback since it is not an automated technique. The repeatability and the intermediate precision of the method were evaluated, as well as the stability of the labeled and non-labeled aSyn samples. After careful screening and optimization of various labeling reagents, 4-fluoro-7-nitrobenzofurazan (N...Continue Reading

References

Sep 7, 1967·Biochemical and Biophysical Research Communications·A L ShapiroJ V Maizel
Oct 29, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·M J SchmerrR C Cutlip
Feb 24, 2001·Annals of the New York Academy of Sciences·K A ConwayP T Lansbury
Mar 22, 2001·Electrophoresis·Y MaE S Yeung
Mar 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Omar M A El-AgnafDavid Allsop
Nov 3, 2006·Electrophoresis·Tai-Chia ChiuHuan-Tsung Chang
Dec 1, 2006·Electrophoresis·Ana M García-CampañaHuguette Fabre
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Clemens R ScherzerMichael G Schlossmacher
Dec 8, 2009·Electrophoresis·Véréna PoinsotFrançois Couderc
Aug 28, 2010·Science·Jacqueline BurréThomas C Südhof
Jan 1, 2010·Journal of Alzheimer's Disease : JAD·Dag SehlinHillevi Englund
Aug 26, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Penelope G FouldsDavid Allsop
Nov 30, 2011·Analytica Chimica Acta·Zaifang ZhuShaorong Liu
Mar 20, 2012·Journal of Neural Transmission·Brit MollenhauerMichael G Schlossmacher
Sep 13, 2012·Electrophoresis·Claudia CianciulliHermann Wätzig
Jan 10, 2013·PloS One·Ana GorostidiOmar M A El-Agnaf
Oct 8, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Nils BolstadKjell Nustad
Apr 20, 2014·Frontiers in Aging Neuroscience·Lucilla ParnettiPaolo Calabresi
May 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Alexander K BuellChristopher M Dobson
Aug 19, 2014·Clinical Neuropathology·Ursula UnterbergerGabor G Kovacs
Oct 14, 2014·Frontiers in Aging Neuroscience·Jan O AaslyOmar M A El-Agnaf
Feb 6, 2017·Journal of Neural Transmission·Ole-Bjørn Tysnes, Anette Storstein
Mar 30, 2017·Biomarkers in Medicine·Naomi P VisanjiUNKNOWN Systemic Synuclein Sampling Study (S4)
Jul 25, 2017·Movement Disorders : Official Journal of the Movement Disorder Society·Brit MollenhauerUNKNOWN Investigating Synuclein Consortium of the Michael J. Fox Foundation for Parkinson's Research
Dec 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Stefano PerniKurt G Beam

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