Separation and determination of coumarins including furanocoumarins using micellar electrokinetic capillary chromatography

Talanta
Sławomir DreslerIreneusz Sowa

Abstract

The conditions of micellar electrokinetic capillary chromatography for separation and simultaneous measurement of coumarins (coumarin, scoparone, isoscopoletin, esculin, esculetin, umbelliferone) including furanocoumarins (xanthotoxin, byakangelicin, isopimpinellin, bergapten, phellopterin, xanthotoxol) have been elaborated. The influence of different parameters, such as the pH of the buffer, sodium cholate (SC) or methanol concentration in the buffer, on the migration time, peak resolution, peak asymmetry, and number of theoretical plates was investigated. The optimum separation of the compounds was achieved using 50-µm i.d. capillaries with a total length of 64.5 cm (56 cm effective length) and a buffer system at pH 9.00 consisting of 50 mM sodium tetraborate, 45 mM SC, and 20% of methanol (v/v). The developed method ensured good repeatability of corrected peak areas and migration times (the relative standard deviations were in the range of 2.8-6.1% and 0.8-4.0%, respectively). The average limit of detection for all studied compounds was below 1.3 µg mL. Moreover, good linearity of the relationship between the peak corrected area and the concentration of the compounds was observed (correlation coefficient >0.99). The method w...Continue Reading

Citations

Apr 22, 2020·Critical Reviews in Analytical Chemistry·Cui Dong-WeiFeng Xue-Song
Jul 31, 2019·Journal of Chromatography. a·Wenbin JinGongke Li
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna PrzybylskaMarcin Koba
May 6, 2021·International Journal of Molecular Sciences·Anna RysiakMagdalena Wójciak
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcin GackowskiArtur Bogacz

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