Separation and determination of flavonoids using microemulsion EKC with electrochemical detection

Electrophoresis
Lishuang YuGuo-Nan Chen

Abstract

A selective and sensitive method of microemulsion EKC (MEEKC) with electrochemical detection (ED) was developed for separation and determination of 14 flavonoids. In order to obtain the better stability for the studied flavonoids, oil (ethyl acetate) with low interfacial surface tension was employed as organic solvent. A running buffer composed of 0.9% (w/v, 30 mM) SDS, 0.9% (w/v, 21 mM) sodium cholate (SC), 0.9% (w/v, 121 mM) butan-1-ol, 0.6% (w/v, 68 mM) ethyl acetate, and 98.2% v/v 10 mM Na(2)B(4)O(7)-20 mM H(3)BO(3) buffer (pH 7.5) was applied for the separation of flavonoids. Under the optimum conditions, the relationship between peak currents and analyte concentrations was linear over about 1.3 and 1.7 orders of magnitude with detection limits (defined as S/N = 3) ranging from 0.02 to 0.5 microg/mL for all analytes. This method was applied for the determination of flavonoids in real samples with simple extraction procedures, and the assay results were satisfactory.

References

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Citations

Dec 25, 2010·Journal of Pharmaceutical and Biomedical Analysis·Roberto Gotti
Aug 21, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zongbao ChenLan Zhang
Dec 25, 2008·Electrophoresis·Richie RyanKevin Altria
Dec 6, 2011·Electrophoresis·Xiao-jia ChenShao-Ping Li
Mar 2, 2010·Electrophoresis·Richie RyanKevin Altria

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