Separation and determination of lipids by one-dimensional micro-thin-layer chromatography followed by densitometry

Journal of Chromatography
W M Frederiks, S Broekhoven

Abstract

A method is described for the rapid analysis of a mixture of phospholipids and neutral lipids, which was used for the analysis of extracts obtained from a nuclear fraction isolated from rat liver. The lipids are separated by one-dimensional thin-layer chromatography on microchromatoplates (48 X 24 mm), using three solvents for development. After spraying the plates with phosphoric acid and heating, the amount of carbon from the charred compounds is measured densitometrically. Only 2-10 microgram of lipid mixture are needed for the determination of the relative amounts of the separated compounds.

References

Sep 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H H Althaus, V Neuhoff
Aug 1, 1964·The Biochemical Journal·C C Widnell, J R Tata
Feb 10, 1970·Biochimica Et Biophysica Acta·A W Konings, F J Loomeijer
Sep 1, 1974·The Journal of Cell Biology·R P Aaronson, G Blobel
Aug 1, 1970·The Journal of Cell Biology·H Kleinig
Apr 1, 1966·Life Sciences·M Song, P Mandel

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Citations

Dec 11, 1978·Journal of Chromatography·F Vitiello, J P Zanetta
Dec 1, 1981·Experimental Eye Research·L Rosenfeld, A Spector
Nov 26, 2015·Analytical Chemistry·Jelle De VosSebastiaan Eeltink

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