Separation and identification of bioactive peptides from stem of Tinospora cordifolia (Willd.) Miers

PloS One
Raman PachaiappanPalaniyandi Velusamy

Abstract

Enzyme hydrolysates (trypsin, papain, pepsin, α-chymotrypsin, and pepsin-pancreatin) of Tinospora cordifolia stem proteins were analyzed for antioxidant efficacy by measuring (1) 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging activity, (2) 2,20-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical scavenging capacity, and (3) Fe2+ chelation. Trypsin hydrolysate showed the strongest DPPH• scavenging, while α-chymotrypsin hydrolysate exhibited the highest ABTS+ scavenging and Fe2+ chelation. Undigested protein strongly inhibited the gastrointestinal enzymes, trypsin (50% inhibition at enzyme/substrate ratio = 1:6.9) and α-chymotrypsin (50% inhibition at enzyme/substrate ratio = 1:1.82), indicating the prolonged antioxidant effect after ingestion. Furthermore, gel filtration purified peptide fractions of papain hydrolysates exhibited a significantly higher ABTS+ and superoxide radical scavenging as compared to non-purified digests. Active fraction 9 showing the highest radical scavenging ability was further purified and confirmed by MALDI-TOF MS followed by MS/MS with probable dominant peptide sequences identified are VLYSTPVKMWEPGR, VITVVATAGSETMR, and HIGININSR. The obtained results revealed that free radica...Continue Reading

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Citations

Jun 20, 2019·Protein and Peptide Letters·Sulochana Priya
Jul 23, 2021·Food Chemistry·Chandana SosalagerePoorva Sharma
Oct 1, 2021·Critical Reviews in Food Science and Nutrition·Vibeke OrlienJorge Ruiz Carrascal

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Datasets Mentioned

BETA
MF459677

Methods Mentioned

BETA
protease assay
RSA
size exclusion chromatography

Software Mentioned

GPS explorer
SPSS Statistics
BLAST
DeNovo explorer
Excel
SPSS

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