Separation and identification of diacylglycerols containing branched chain fatty acids by liquid chromatography-mass spectrometry

Journal of Chromatography. a
Andrea Palyzová, Tomáš Řezanka

Abstract

A combination of two chromatographic and two enzymatic methods was used for the analysis of molecular species of lipids from Gram-positive bacteria of the genus Kocuria. Gram-positive bacteria contain a majority of branched fatty acids (FAs), especially iso- and/or anteiso-FAs. Two strains K. rhizophila were cultivated at three different temperatures (20, 28, and 37°C) and the majority phospholipid, i.e., the mixture of molecular species of phosphatidylglycerols (PGs) was separated by means of hydrophilic interaction liquid chromatography (HILIC). After enzymatic hydrolysis of PGs by phospholipase C and derivatization of the free OH group, the sn-1,2-diacyl-3-acetyl triacylglycerols (AcTAGs) were separated by reversed phase HPLC. Molecular species such as i-15:0/i-15:0/2:0, ai-15:0/ai-15:0/2:0, and 15:0/15:0/2:0 (straight chains) were identified by liquid chromatography-positive electrospray ionization mass spectrometry. The tandem mass spectra of both standards and natural compounds containing iso, anteiso and straight chain FAs with the same carbons were identical. Therefore, for identification of the ratio of two regioisomers, i.e. i-15:0/ai-15:0/2:0 vs. ai-15:0/i-15:0/2:0, they were cleavage by pancreatic lipase. The mixtur...Continue Reading

References

Mar 1, 1980·Journal of Bacteriology·K YokotaM Kito
Jul 17, 1998·Biochimica Et Biophysica Acta·R Koynova, M Caffrey
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
May 18, 2005·Methods : a Companion to Methods in Enzymology·Arnis Kuksis, Yutaka Itabashi
Mar 28, 2006·Microbiological Research·Marina TeminaValery M Dembitsky
May 5, 2007·Rapid Communications in Mass Spectrometry : RCM·Delphine OurselCatherine M Lange
Feb 12, 2008·Nature Reviews. Microbiology·Yong-Mei Zhang, Charles O Rock
Oct 21, 2011·Antonie van Leeuwenhoek·Monu BalaShanmugam Mayilraj
Nov 21, 2013·International Journal of Systematic and Evolutionary Microbiology·Syed G DastagerWen-Jun Li
May 17, 2014·The British Journal of Nutrition·Rinat Rivka Ran-ResslerJ Thomas Brenna
Sep 13, 2015·Antonie van Leeuwenhoek·Zhao JiangWen-Jun Li
Dec 3, 2016·International Journal of Systematic and Evolutionary Microbiology·Limin ZhangYing Huang
Dec 18, 2018·International Journal of Systematic and Evolutionary Microbiology·Markus Santhosh BraunMichael Wink
Dec 24, 2018·Analytical and Bioanalytical Chemistry·Katharina WoznyBritta Brügger
May 16, 2019·International Journal of Systematic and Evolutionary Microbiology·Li TuoCheng-Hang Sun

❮ Previous
Next ❯

Citations

May 21, 2021·Journal of the American Society for Mass Spectrometry·Christian FreemanKelly M Hines

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology
A G O'DonnellK P Schaal
International Journal of Systematic and Evolutionary Microbiology
Olga I NedashkovskayaKyung Sook Bae
International Journal of Systematic and Evolutionary Microbiology
Elena P IvanovaRichard Christen
© 2021 Meta ULC. All rights reserved