Separation and Loss of Centrioles From Primordidal Germ Cells To Mature Oocytes In The Mouse

Scientific Reports
C SimerlyG Schatten

Abstract

Oocytes, including from mammals, lack centrioles, but neither the mechanism by which mature eggs lose their centrioles nor the exact stage at which centrioles are destroyed during oogenesis is known. To answer questions raised by centriole disappearance during oogenesis, using a transgenic mouse expressing GFP-centrin-2 (GFP CETN2), we traced their presence from e11.5 primordial germ cells (PGCs) through oogenesis and their ultimate dissolution in mature oocytes. We show tightly coupled CETN2 doublets in PGCs, oogonia, and pre-pubertal oocytes. Beginning with follicular recruitment of incompetent germinal vesicle (GV) oocytes, through full oocyte maturation, the CETN2 doublets separate within the pericentriolar material (PCM) and a rise in single CETN2 pairs is identified, mostly at meiotic metaphase-I and -II spindle poles. Partial CETN2 foci dissolution occurs even as other centriole markers, like Cep135, a protein necessary for centriole duplication, are maintained at the PCM. Furthermore, live imaging demonstrates that the link between the two centrioles breaks as meiosis resumes and that centriole association with the PCM is progressively lost. Microtubule inhibition shows that centriole dissolution is uncoupled from micro...Continue Reading

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Citations

Sep 26, 2020·Frontiers in Cell and Developmental Biology·Maíra Bianchi Rodrigues AlvesClémence Belleannée
Feb 10, 2021·Theriogenology·Ying XuHai-Long Wang
Jul 28, 2021·Annual Review of Cell and Developmental Biology·Sónia Gomes PereiraMónica Bettencourt-Dias
Oct 29, 2021·The Biochemical Journal·Ieng Fong SouUrszula Lucja McClurg

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