Separation and preparation of 6-gingerol from molecular distillation residue of Yunnan ginger rhizomes by high-speed counter-current chromatography and the antioxidant activity of ginger oils in vitro

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Zhilin GanYuanying Ni

Abstract

Molecular distillation residue (MD-R) from ginger had the most total phenol content of 247.6mg gallic acid equivalents per gram (GAE/g) among the ginger oils. High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale was successfully performed in separation and purification of 6-gingerol from MD-R by using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (10:2:5:7, v/v/v/v). The target compound was isolated, collected, purified by HSCCC in the head-tail mode, and then analyzed by HPLC. A total of 90.38±0.53mg 6-gingerol was obtained from 600mg MD-R, with purity of 99.6%. In addition, the structural identification of 6-gingerol was performed by EI/MS, (1)H NMR and (13)C NMR. Moreover, the orders of antioxidant activity were vitamin E (VE)>supercritical fluid extraction oleoresin (SFE-O)=MD-R=6-gingerol>molecular distillation essential oil (MD-EO) and butylated hydroxytoluene (BHT)=VE>6-gingerol>MD-R=SFE-O>MD-EO, respectively in 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging and β-Carotene bleaching.

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Citations

Jun 23, 2016·Journal of Pharmaceutical and Biomedical Analysis·Hongli JinXinmiao Liang
Jul 20, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiping DuHui Ni
May 10, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lei WenDaijie Wang
May 26, 2018·International Journal of Molecular Sciences·Nicholas Ekow ThomfordKevin Dzobo
Sep 9, 2021·Clinical Microbiology Reviews·Oyelola AdegboyeClaire Loiseau
Oct 1, 2021·International Journal of Oral Science·Qiong ZhangJing Zou

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