Separation and purification of epigallocatechin-3-gallate (EGCG) from green tea using combined macroporous resin and polyamide column chromatography

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Xin JinMinjie Wei

Abstract

Epigallocatechin-3-gallate (EGCG) is a major bioactive ingredient of green tea that produces beneficial neuroprotective effects. In this paper, to optimize the EGCG enrichment, thirteen macroporous resins with different chemical and physical properties were systemically evaluated. Among the thirteen tested resins, the H-bond resin HPD826 exhibited best adsorption/desorption capabilities and desorption ratio, as well as weakest affinity for caffeine. The absorption of EGCG on the HPD826 resin followed the pseudo-second-order kinetics and Langmuir isotherm model. The separation parameters of EGCG were optimized by dynamic adsorption/desorption experiments with the HPD826 resin column. Under the optimal condition, the content of EGCG in the 30% ethanol eluent increased by 5.8-fold from 7.7% to 44.6%, with the recovery yield of 72.1%. After further purification on a polyamide column, EGCG with 74.8% purity was obtained in the 40-50% ethanol fraction with a recovery rate of 88.4%. In addition, EGCG with 95.1% purity could be easily obtained after one-step crystallization in distilled water. Our study suggests that the combined macroporous resin and polyamide column chromatography is a simple method for large-scale separation and pur...Continue Reading

References

Aug 9, 2005·Chemosphere·Erol Ayranci, Numan Hoda
Sep 16, 2009·The British Journal of Nutrition·Rosalind J MooreAnne M Minihane
Nov 12, 2010·Journal of Bioscience and Bioengineering·Jian-Hui YeYue-Rong Liang
Nov 26, 2010·Archives of Biochemistry and Biophysics·Santosh K Katiyar
Apr 17, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ya-Jun WangYin-Chu Shen
Sep 29, 2012·The Journal of Nutritional Biochemistry·Sung Un KangChul-Ho Kim
Oct 9, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhanquan DuFeng Qiu
Oct 24, 2012·Journal of Chromatography. a·Shaoyan WangSiwen Zheng
Feb 16, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Palaniswamy KalaiselviViswanadha Vijaya Padma
Dec 30, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shu-Ping ZouYin-Chu Shen

❮ Previous
Next ❯

Citations

Jan 29, 2021·Analytical Methods : Advancing Methods and Applications·Li WangHongxin Wang
May 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Mehdi NikooHassan Ahmadi Gavlighi
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoya WangXiang Fu
Oct 28, 2021·Critical Reviews in Food Science and Nutrition·Hang LiRen-You Gan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.