SEPARATION AND PURIFICATION OF TWO MINOR COMPOUNDS FROM RADIX ISATIDIS BY INTEGRATIVE MPLC AND HSCCC WITH PREPARATIVE HPLC

Journal of Liquid Chromatography & Related Technologies
Zhenjie LiangYoichiro Ito

Abstract

Radix isatidis has been widely used as a Chinese traditional medicine for its anti-virus and anticancer activities where the minor components may contribute to these beneficial pharmaceutical effects. In order to enrich the target minor compounds effectively and rapidly, extraction, medium-pressure liquid chromatography (MPLC), high-speed countercurrent chromatography (HSCCC) and preparative high-performance liquid chromatography (pre-HPLC) were integratively used for separation and purification of two target minor compounds indole-3-acetonitrile-6-O-β-D-glucopyranoside (target 1) and clemastanin B (target 2) in the present study. Radix isatidis was dried, pulverized and extracted with 50% methanol at room temperature, then concentrated and subjected to pretreatment with D-101 macroporous resin chromatography and extraction by MPLC. The first target compound was separated by MPLC at the purity raised to 70-80%, but without the second minor compounds which were irreversibly adsorbed by C18 solid support. Therefore, the second target compound in the crude extract was directly separated by HSCCC at purity of 80-90%. Finally these refined samples were further separated by pre-HPLC to obtain a high purity at 98-99%. The chemical str...Continue Reading

References

Apr 22, 2005·Acta Pharmacologica Sinica·Jian-guo FangHong-gang Han
Aug 4, 2006·Journal of Pharmaceutical and Biomedical Analysis·Bing-Chung LiauShih-Shiung Chen
Jul 22, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei-Jun KongWei-Ying Guo
Apr 20, 2010·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Hong-Wei LiShou-Kai Jia
May 10, 2011·Journal of Liquid Chromatography & Related Technologies·Xiaohong WangYoichiro Ito
Nov 18, 2011·Analytical and Bioanalytical Chemistry·Xiuli ZhangXinmiao Liang

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