Separation and Quantification of Four Main Chiral Glucosinolates in Radix Isatidis and Its Granules Using High-Performance Liquid Chromatography/Diode Array Detector Coupled with Circular Dichroism Detection

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yanhong ShiRui Wang

Abstract

As chemical drugs, separation and quantification of the specific enantiomer from the chiral compounds in herbal medicines are becoming more important. To clarify the chemical characterization of chiral glucosinolates-the antiviral active ingredients of Radix Isatidis, an optimized efficient method of HPLC-UV-CD was developed to simultaneously separate and quantify the four main chiral glucosinolates: progoitrin, epiprogoitrin, and R,S-goitrin. The first step was to determine progoitrin, epiprogoitrin, and R,S-goitrin using HPLC-UV, and then determine the R-goitrin and S-goitrin by coupling with CD detection. Subsequently, through the linear relations between anisotropy factor (g factor) and the percent optical purity of R-goitrin, the contents of R-goitrin and S-goitrin from the R,S-goitrin mixture were calculated separately. Furthermore, the chemical composition features of the four chiral glucosinolates in 37 samples from crude drugs, decoction pieces, and granules of R. Isatidis were conducted. The total content of the four glucosinolates was obviously higher in crude drugs, and the variance character of each glucosinolate contents was different. In summary, the accurate measurement method reported here allows for better con...Continue Reading

References

Oct 3, 2002·Nature Reviews. Drug Discovery·Israel AgranatJohn Caldwell
Oct 26, 2011·Journal of Agricultural and Food Chemistry·Zhiyong XieYiming Li
Aug 23, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Shu-Jen ChangCheng-Wen Lin
Feb 4, 2016·Nature Reviews. Drug Discovery·Asher Mullard
Mar 30, 2016·Journal of Analytical Methods in Chemistry·Lixing NieShuangcheng Ma
Sep 25, 2017·Journal of Pharmaceutical and Biomedical Analysis·Andrea Calcaterra, Ilaria D'Acquarica

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Methods Mentioned

BETA
X-ray
circular dichroism

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