Separation and structure-function studies of Taiwan cobra cardiotoxins

Journal of Protein Chemistry
Shinne-Ren LinKee-Lung Chang

Abstract

Six cardiotoxins (CTXs) and one cardiotoxin-like basic protein (CLBP) from Naja naja atra (Taiwan cobra) venom were separated by a SP-Sephadex C-25 column. CTXn and CTXI were well separated by eluting with ammonium acetate buffer, and the separation of CLBP from CTXIV and CTXV mixtures was achieved using sodium phosphate buffer. These findings suggest a differential interaction of CTXs with the chromatographic matrix using different buffer systems. Chemical modification studies on cationic residues of CTXI suggested that there was no single lysine or arginine residue exclusively responsible for its biological activity. Moreover, it was found that the cytotoxicity and hemolytic sites of CTXI could be dissociated by chemical modifications. It suggests the potentiality for preparing toxin derivatives in which a specific activity is retained.

Citations

Aug 12, 2004·Biological & Pharmaceutical Bulletin·Heng ZhouRongqing Zhang
Dec 7, 2006·Molecular and Cellular Biochemistry·Sheng-Huei YangShinne-Ren Lin
Jun 19, 2008·Journal of Biochemical and Molecular Toxicology·Sheng-Huei YangShinne-Ren Lin
May 29, 2008·Clinical and Experimental Pharmacology & Physiology·Ching-Ming ChienShinne-Ren Lin
Apr 2, 2009·Biological & Pharmaceutical Bulletin·Xing-Yong ChenKang-Sen Xu
May 22, 2007·Cell Biochemistry and Function·Ching-Ming ChienShinne-Ren Lin
Jul 20, 2005·Clinical and Experimental Pharmacology & Physiology·Sheng-Huei YangShinne-Ren Lin
Feb 21, 2006·Clinical and Experimental Pharmacology & Physiology·Chia-Houng TsaiShinne-Ren Lin
Feb 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi-Jun ShiLong-Sen Chang
Aug 19, 2015·Journal of Biochemical and Molecular Toxicology·Pei-Chien TsaiShinne-Ren Lin
Jun 27, 2014·Cell Biochemistry and Function·Pei-Chien TsaiShinne-Ren Lin
Jun 15, 2019·International Journal of Biological Macromolecules·Yi-Jun ShiLong-Sen Chang
Jun 9, 2004·Biochemical and Biophysical Research Communications·Ya-Ling LinLong-Sen Chang
Mar 15, 2005·Life Sciences·Sheng-Huei YangShinne-Ren Lin
Aug 11, 2006·Toxicon : Official Journal of the International Society on Toxinology·Long-Sen ChangHsien-Bin Huang
Mar 21, 2007·Toxicon : Official Journal of the International Society on Toxinology·Sheng-Huei YangShinne-Ren Lin
Jan 29, 2008·Toxicon : Official Journal of the International Society on Toxinology·Ku-Chung ChenLong-Sen Chang
Feb 11, 2010·Toxicon : Official Journal of the International Society on Toxinology·Kuei-Li LinShinne-Ren Lin

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