Dec 7, 2019

Separation-Free Electrogenerated Chemiluminescence Immunoassay Incorporating Target Assistant Proximity Hybridization and Dynamically Competitive Hybridization of a DNA Signal Probe

Analytical Chemistry
Bing WangChengxiao Zhang

Abstract

A separation-free electrogenerated chemiluminescence (ECL) immunoassay for biomarkers has been developed incorporating target assistant proximity hybridization and dynamically competitive hybridization of a DNA ECL signal probe for the first time. In this work, the biomarkers of acute myocardial infarction including cardiac troponin I (cTnI), cardiac troponin T (cTnT), and myoglobin (Myo) were chosen as the model proteins while the corresponding antibody was utilized as a recognition probe and the DNA5 tagged with ruthenium complex was chosen as an ECL signal probe (DNA5-Ru1). The biosensors were fabricated by covalently coupling the capture probe DNA1 onto the surface of a glassy carbon electrode, and then, a competitor ss-DNA2 was hybridized with DNA1. When the biosensor was incubated in the solution containing a target protein, the recognition probes (DNA3-Ab1 and DNA4-Ab2), DNA5-Ru1, and the coreactant tri-n-propylamine, the target protein was bounded with two antibodies of the recognition probes and thus induced the sufficient proximity hybridization of DNA3 with DNA1, DNA4 with DNA5-Ru1, and DNA5-Ru1 with DNA1 and the unwinding of the competitor DNA2 with DNA1, and ECL measurement was performed in separation-free format. ...Continue Reading

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Mentioned in this Paper

Biological Markers
Troponin
Troponin I
Troponin T, Cardiac Muscle
SFMBT1
Carbon
TNNI3
Recognition (Psychology)
Myoglobin
Heart

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