Separation in slalom chromatography: stretching and velocity dependence

Analytical Chemistry
Y C GuillaumeMireille Thomassint

Abstract

Slalom chromatography (SC) is used for the separation of large double-stranded DNA molecules. In this technique, the progression of the DNA fragments through the closed column packing follows the flow direction and is like a snake edging is way into long grass. A novel mathematical model is developed in this paper to describe this hydrodynamic phenomenon. The results obtained provided a model for the resolution between two adjacent peaks on a chromatogram. As well, a chromatographic response function was used to obtain the most efficient separation conditions for a mixture of DNA fragments with sizes higher than 15 kbp in a minimum analysis time.

References

Aug 25, 1993·Journal of Chromatography·K Kasai
Aug 19, 2000·Journal of Chromatography. a·E PeyrinA Favier

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Citations

Sep 10, 2004·Journal of Separation Science·Mojca BencinaAles Strancar

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