Separation mechanism of pullulan solution-filled capillary electrophoresis of sodium dodecyl sulfate-proteins

Electrophoresis
M NakataniT Nakagawa

Abstract

The separation mechanism of capillary electrophoresis of sodium dodecyl sulfate (SDS)-proteins using pullulan with a molecular mass range of 50,000-100,000 as a separation matrix was investigated. The pullulan solution was filled into fused-silica capillaries whose inner walls were coated with linear polyacrylamide through chemically stable Si-C linkages. Baseline separations of SDS proteins were achieved at concentrations ranging from 3-10% w/v of pullulan. The entanglement threshold of pullulan solution was found to be around 0.5% w/v, indicating migration of SDS-proteins through an entangled pullulan network. Ferguson plots exhibited a linear relationship between log mobility and pullulan concentration. Linear relationships were also obtained for double logarithmic plots of the electrophoretic mobility and protein molecular mass. These results show that the separation is based on mass discrimination in accordance with the Ogston theory.

References

Jan 1, 1992·Annual Review of Physical Chemistry·J Noolandi
Feb 1, 1992·Genetic Analysis, Techniques and Applications·P D GrossmanD Hershey
Aug 11, 1989·Journal of Chromatography·B L KargerA Guttman
Oct 21, 1983·Science·J W Jorgenson, K D Lukacs
Aug 1, 1995·Electrophoresis·M NakataniT Nakagawa
Oct 14, 1988·Science·M J GordonR N Zare

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Citations

Mar 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroki OkadaYoshinobu Baba
Sep 10, 2014·The Analyst·Minsub ChungAmy E Herr
Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·D Corradini
Feb 5, 2008·Electrophoresis·Gloria OliverAnuj Chauhan
Jun 17, 2006·Biomedical Chromatography : BMC·Ivan MiksíkTibor Horváth
Jul 30, 2011·Applied Microbiology and Biotechnology·Kuan-Chen ChengJeffrey M Catchmark

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