PMID: 7526903Jul 1, 1994Paper

Separation of 32P-postlabeled DNA adducts of polycyclic aromatic hydrocarbons and nitrated polycyclic aromatic hydrocarbons by HPLC

Chemical Research in Toxicology
Leon C KingJ Lewtas

Abstract

The 32P-postlabeling assay, thin-layer chromatography, and reverse-phase high-pressure liquid chromatography (HPLC) were used to separate DNA adducts formed from 10 polycyclic aromatic hydrocarbons (PAHs) and 6 nitrated polycyclic aromatic hydrocarbons (NO2-PAHs). The PAHs included benzo[j]fluoranthene, benzo[k]fluoranthene, indeno[1,2,3-cd]pyrene, benzo[a]pyrene, chrysene, 6-methylchrysene, 5-methylchrysene, and benz[a]anthracene. The NO2-PAHs included 1-nitropyrene, 2-nitrofluoranthene, 3-nitrofluoranthene, 1,6-dinitropyrene, 1,3-dinitropyrene, and 1,8-dinitropyrene. Separation of seven of the major PAH-DNA adducts was achieved by an initial PAH HPLC gradient system. The major NO2-PAH-DNA adducts were not all separated from each other using the initial PAH HPLC gradient but were clearly separated from the PAH-DNA adducts. A second NO2-PAH HPLC gradient system was developed to separate NO2-PAH-DNA adducts following one-dimensional TLC and HPLC analysis. HPLC profiles of NO2-PAH-DNA adducts were compared using both adduct enhancement versions of the 32P-postlabeling assay to evaluate the use of this technique on HPLC to screen for the presence of NO2-PAH-DNA adducts. To demonstrate the application of these separation methods to...Continue Reading

Citations

Sep 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C M Dale, R C Garner
May 8, 2007·Cancer Research·Carol S TrempusRaymond W Tennant
May 1, 1996·Environmental Health Perspectives·K SavelaJ Lewtas
Sep 15, 2010·Environmental and Molecular Mutagenesis·M Margaret PrattMiriam C Poirier
Jun 7, 2014·Environmental Science and Pollution Research International·Masoud NaseriJavad Barabady

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