Abstract
Compared with most penicillin-susceptible isolates of Streptococcus pneumoniae, penicillin-resistant clinical isolate Hun 663 contains mosaic penicillin-binding protein (PBP) genes encoding PBPs with reduced penicillin affinities, anomalous molecular sizes, and also cell walls of unusual chemical composition. Chromosomal DNA prepared from Hun 663 was used to transform susceptible recipient cells to donor level penicillin resistance, and a resistant transformant was used next as the source of DNA in the construction of a second round of penicillin-resistant transformants. The greatly reduced penicillin affinity of the high-molecular-weight PBPs was retained in all transformants through both genetic crosses. On the other hand, PBP pattern and abnormal cell wall composition, both of which are stable, clone-specific properties of strain Hun 663, were changed: individual transformants showed a variety of new, abnormal PBP patterns. Furthermore, while the composition of cell walls resembled that of the DNA donor in the first-round transformants, it became virtually identical to that of susceptible pneumococci in the second-round transformants. The findings indicate that genetic elements encoding the low affinity of PBPs and the penic...Continue Reading
References
Jul 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R MunozA Tomasz
Nov 1, 1991·Journal of Bacteriology·G Laible, R Hakenbeck
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J Garcia-Bustos, A Tomasz
Jan 1, 1985·Molecular & General Genetics : MGG·E GarcíaR López
Jan 1, 1989·The Journal of Infectious Diseases·D JabesA Tomasz
Jan 1, 1986·The Journal of Infectious Diseases·S Handwerger, A Tomasz
Mar 1, 1980·Antimicrobial Agents and Chemotherapy·S Zighelboim, A Tomasz
Mar 1, 1980·Antimicrobial Agents and Chemotherapy·R HakenbeckA Tomasz
Oct 1, 1980·Antimicrobial Agents and Chemotherapy·R WilliamsonA Tomasz
Jan 1, 1996·Journal of Bacteriology·A Severin, A Tomasz
Apr 1, 1995·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·T J CoffeyB G Spratt
Apr 22, 1960·Biochimica Et Biophysica Acta·S LACKS, R D HOTCHKISS
Citations
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·J van Heijenoort, L Gutmann
May 10, 2017·Antimicrobial Agents and Chemotherapy·Inga SchweizerDalia Denapaite
Nov 7, 2008·The Journal of Biological Chemistry·Ailsa J PowellChristopher Davies
Feb 6, 2008·FEMS Microbiology Reviews·André ZapunThierry Vernet
Jul 12, 2017·Frontiers in Cellular and Infection Microbiology·Colette G Ngo NdjomHarlan P Jones
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·S R Filipe, A Tomasz
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·N MouzO Dideberg
Mar 21, 2002·Expert Opinion on Therapeutic Targets·David W Green
Jun 12, 2003·The Journal of Biological Chemistry·Aude AntignacMuhamed-Kheir Taha
Feb 2, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·S R FilipeA Tomasz
Mar 8, 2012·Future Microbiology·Regine HakenbeckPatrick Maurer
Sep 13, 2001·The Journal of Biological Chemistry·A DessenO Dideberg
Mar 21, 2003·Protein Engineering·Mariana Peimbert, Lorenzo Segovia
Aug 28, 2001·The Journal of Biological Chemistry·S R FilipeA Tomasz
Nov 14, 2000·Journal of Bacteriology·S R FilipeA Tomasz
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·S Rohrer, B Berger-Bächi
Apr 22, 2003·Infection and Immunity·Arun S Kharat, Alexander Tomasz
Mar 10, 2009·Journal of Bacteriology·Skye M BarendtMalcolm E Winkler
Jun 28, 2000·The Journal of Biological Chemistry·S R FilipeA Tomasz
Apr 16, 1998·Journal of Bacteriology·R HakenbeckL Gutmann
Jun 22, 2000·Journal of Molecular Biology·E GordonO Dideberg
May 1, 1997·Antimicrobial Agents and Chemotherapy·J Krauss, R Hakenbeck
Jun 8, 2019·Microbiology Spectrum·Waldemar VollmerAlexander Tomasz
Aug 1, 1997·Journal of Bacteriology·G ZhaoS R Jaskunas