PMID: 8606149Apr 1, 1996Paper

Separation of abnormal cell wall composition from penicillin resistance through genetic transformation of Streptococcus pneumoniae

Journal of Bacteriology
A SeverinA Tomasz

Abstract

Compared with most penicillin-susceptible isolates of Streptococcus pneumoniae, penicillin-resistant clinical isolate Hun 663 contains mosaic penicillin-binding protein (PBP) genes encoding PBPs with reduced penicillin affinities, anomalous molecular sizes, and also cell walls of unusual chemical composition. Chromosomal DNA prepared from Hun 663 was used to transform susceptible recipient cells to donor level penicillin resistance, and a resistant transformant was used next as the source of DNA in the construction of a second round of penicillin-resistant transformants. The greatly reduced penicillin affinity of the high-molecular-weight PBPs was retained in all transformants through both genetic crosses. On the other hand, PBP pattern and abnormal cell wall composition, both of which are stable, clone-specific properties of strain Hun 663, were changed: individual transformants showed a variety of new, abnormal PBP patterns. Furthermore, while the composition of cell walls resembled that of the DNA donor in the first-round transformants, it became virtually identical to that of susceptible pneumococci in the second-round transformants. The findings indicate that genetic elements encoding the low affinity of PBPs and the penic...Continue Reading

References

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Mar 1, 1980·Antimicrobial Agents and Chemotherapy·S Zighelboim, A Tomasz
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Citations

May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·J van Heijenoort, L Gutmann
Feb 6, 2008·FEMS Microbiology Reviews·André ZapunThierry Vernet
Jul 12, 2017·Frontiers in Cellular and Infection Microbiology·Colette G Ngo NdjomHarlan P Jones
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·S R Filipe, A Tomasz
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·N MouzO Dideberg
Mar 21, 2002·Expert Opinion on Therapeutic Targets·David W Green
Feb 2, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·S R FilipeA Tomasz
Mar 8, 2012·Future Microbiology·Regine HakenbeckPatrick Maurer
Mar 21, 2003·Protein Engineering·Mariana Peimbert, Lorenzo Segovia
Jun 8, 2019·Microbiology Spectrum·Waldemar VollmerAlexander Tomasz

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