Separation of amphipathic proteins based on adsorption to hexadecane:water interfaces

Preparative Biochemistry
M RosenbergI Eli

Abstract

A method is proposed for the relatively simple and rapid separation of amphipathic biopolymers, based on adsorption onto and desorption from the surface of hexadecane droplets. Adsorption to the hexadecane:water interface was carried out by mixing hexadecane with aqueous protein solutions at room temperature. Desorption was performed by consecutive solidification and melting of the liquid hydrocarbon (m.p. 18 degrees C), resulting in coalescence of the droplets and reappearance of the desorbed moiety in the bulk aqueous phase. Of interest was the observation that lysozyme remains enzymatically active following this procedure.

References

Jan 1, 1984·Methods in Enzymology·S Shaltiel
Oct 1, 1981·Journal of Bacteriology·M Rosenberg, E Rosenberg

Citations

Aug 23, 2006·FEMS Microbiology Letters·M Rosenberg
Jan 1, 1991·Critical Reviews in Microbiology·M Rosenberg

Related Concepts

n-hexadecane
Adsorption
Alkanes
Leftose
Saliva
Structure-Activity Relationship

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