Separation of barbiturates and phenylthiohydantoin amino acids using the thermally tuned tandem column concept

Analytical Chemistry
Y Mao, Peter W Carr

Abstract

There are many more choices of column type than of eluent type for method development in reversed-phase liquid chromatography. It is common to switch between different column types or between the same type from different suppliers to achieve the desired separations. The key difficulty in modulating band spacing by adjusting the column type is that it is a discontinuous, "hit or miss" proposition. The thermally tuned tandem column (T3C) concept effectively solves this problem by connecting two columns in series and independently controlling the two column temperatures. The columns are chosen to have distinctively different chromatographic selectivities (band spacing), so that the unresolved peaks on one column are separated by the other. The optimized separation in the T3C is achieved by simultaneously tuning the two column temperatures. In this study, we used the T3C combination of a carbon and a conventional bonded phase for the separation of barbiturates and phenylthiohydantoin amino acids (PTH-amino acids). Good peak shapes and comparable retention times were observed on the two phases at room temperature. The selectivities on the two phases are quite different. Baseline separations were easily achieved with the T3C set alth...Continue Reading

References

Feb 7, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·L R Snyder

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Citations

Feb 19, 2004·Journal of Chromatography. a·J NawrockiP W Carr
Feb 28, 2002·Carbohydrate Research·Guangli YuRobert J Linhardt
May 9, 2016·Analytica Chimica Acta·T Alvarez-SeguraM C García-Alvarez-Coque
Jan 5, 2011·Analytical Chemistry·Deirdre CabooterGert Desmet
Aug 30, 2002·Analytical Chemistry·Jonathan D Thompson, Peter W Carr

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