PMID: 6404244Feb 1, 1983Paper

Separation of beta-D-galactosidases in rabbit tissues: genetics of neutral beta-D-galactosidase

Biochemical Genetics
L F van ZutphenR R Fox

Abstract

Three different types of beta-D-galactosidase (EC 3.2.1.23) could be distinguished in rabbit tissues using electrophoretic procedures. (1) Acid beta-D-galactosidase with a low mobility and maximal activity at pH 3-5 was found in the particulate fraction of various tissue homogenates. This enzyme hydrolyzed 4-methylumbelliferyl-D-galactoside, but no activity against other glycoside substrates could be demonstrated. The enzyme was inhibited by galactono-(1 leads to 4)-lactone. (2) Lactose-hydrolyzing beta-D-galactosidase with an intermediate mobility was found only in juvenile small intestine. Most of the activity was found in the particulate fraction of the cell. The enzyme hydrolyzed several other synthetic glycoside substrates besides lactose. It was most active at pH 5-6 and strongly inhibited by glucono-(1 leads to 5)-lactone but not much affected by galactono-(1 leads to 4)-lactone. (3) Neutral beta-D-galactosidase with a fast mobility and maximal activity at pH 6-8 was found in the soluble fraction of homogenates from liver, kidney, and small intestine. This enzyme also showed a broad substrate specificity; it possessed activity against aryl-beta-D-glucoside, -fucoside, and -galactoside substrates but not against lactose. ...Continue Reading

References

Jan 23, 1976·Biochimica Et Biophysica Acta·R H GlewA R Christopher
Sep 15, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·B K BurtonH L Nadler
Jan 1, 1979·Annual Review of Genetics·K Paigen
Apr 9, 1977·The Veterinary Record·W J Donnelly, M Kelly
Nov 17, 1975·Biochemical and Biophysical Research Communications·J N KanferD S Layne
Apr 8, 1976·Biochimica Et Biophysica Acta·M A ChesterP A Ockerman
Apr 1, 1972·Journal of Comparative Pathology·W F Blakemore
Mar 1, 1969·The Biochemical Journal·H E Abrahams, D Robinson

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Citations

Jan 1, 1986·The International Journal of Biochemistry·A Jauhiainen, T Vanha-Perttula
Oct 28, 2008·Biochimica Et Biophysica Acta·Yoko Funakoshi, Tadashi Suzuki
Mar 23, 2011·Biochemistry. Biokhimii︠a︡·S S ShishkinI N Krakhmaleva

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