Separation of cephalosporins on thin silica gel layers impregnated with transition metal ions and by reversed-phase TLC

Biomedical Chromatography : BMC
R Bhushan, G Thuku Thiong'o

Abstract

Cephalosporin antibiotics were separated on thin layer plates impregnated with transition metal ions, Mn(2+), Fe(2+), Co(2+), Ni(2+) and Cu(2+), using different concentrations. Various solvent systems were developed for the study and were used for separation of these analytes. Impregnation was observed to have an effect on hR(F) values, removed tailing of analytes and improved the resolution. The results have been discussed for each metal ion and compared, and the best conditions of separation have been identified. Activation time of thin layer plates impregnated with 0.1% FeSO(4) was found to affect both hR(F) values and resolution of cephalosporins. New solvent systems are reported for both normal phase and reversed-phase TLC.

References

Jun 15, 1992·Analytical Chemistry·J Sherma
Jun 15, 1994·Analytical Chemistry·J Sherma
Sep 1, 1996·Biomedical Chromatography : BMC·R Bhushan, V Parshad
Jul 1, 1998·Biomedical Chromatography : BMC·D AgbabaS Vladimirov
Jul 6, 2000·Analytical Chemistry·J Sherma
Jun 1, 1990·Analytical Chemistry·J Sherma
Jun 1, 1990·Analytical Chemistry·P M Cooke

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Citations

Sep 21, 2010·Biomedical Chromatography : BMC·Dhruv K Singh, Gunjan Maheshwari
Aug 11, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Dhruv K Singh, Gunjan Maheshwari
Jun 15, 2004·Analytical Chemistry·Joseph Sherma

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