PMID: 8586066Oct 1, 1995Paper

Separation of cerebrospinal fluid proteins using capillary electrophoresis: a potential method for the diagnosis of neurological disorders

Electrophoresis
G CowdreyG Firth

Abstract

A method for the analysis of proteins in cerebrospinal fluid (CSF) using free solution capillary electrophoresis (FSCE) was developed, in which the adsorption of proteins to the fused silica capillary wall was virtually eliminated. FSCE was carried out on 30 randomly selected CSF samples. Good sensitivity and resolution was obtained with minimal baseline noise. These preliminary results clearly demonstrated the potential of FSCE to analyse proteins in CSF, both qualitatively and quantitatively more rapidly than has been possible hitherto. A total run time of approximately 30 min allowed between 20 to 25 peaks to be separated. Many peaks with long migration times were detected in CSF that appeared to be specific since they were not detectable in corresponding serum samples. The separation patterns for most of the CSF samples selected showed relatively similar patterns but there were often minor qualitative and quantitative differences. Four CSF samples showed patterns that were significantly different from the others, primarily in the peaks with long migration times. A larger clinical study is now being undertaken.

Citations

May 19, 2001·Electrophoresis·C J StewartD Perrett
Feb 10, 1998·Electrophoresis·V Dolnik
Mar 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·John R PetersenDeborah A Payne
Oct 29, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·R LehmannH M Liebich
Jan 19, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·M A Jenkins
Jan 24, 2004·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Gordana Zunić
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Jul 17, 2012·Journal of Chromatography. a·Petr KubáňMihkel Kaljurand
Apr 1, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David K Lloyd
Nov 20, 1998·Experimental Eye Research·G CowdreyG Firth
Jun 15, 1997·Analytical Chemistry·D J AndersonK A Davis
Jun 17, 1999·Annals of Clinical Biochemistry·D Perrett

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