Separation of chemical warfare agent degradation products by the reversal of electroosmotic flow in capillary electrophoresis

Analytical Chemistry
A E NassarL D Hoffland

Abstract

We report the development of analyses for nerve agent degradation products or related species by the reversal of electroosmotic flow in capillary electrophoresis (CE). The developed methods were used in this laboratory for analysis of samples in the second and third official proficiency tests (International Round-Robins) for the Provisional Technical Secretariat/Preparatory Commission for the Organization for the Prohibition of Chemical Weapons, and those results are reported here. Analytes studied include methylphosphonic acid (a dibasic acid), the monoisopropyl ester of ethylphosphonic acid, and the monoalkyl esters of methylphosphonic acid (R = ethyl, isopropyl, isobutyl, pinacolyl (3,3-dimethyl-2-butyl), cyclohexyl, and 2-ethylhexyl). The cationic surfactants used here for the reversal of electroosmotic flow are didodecyldimethylammonium hydroxide and cetyltrimethylammonium hydroxide. CE methods using conductivity or indirect UV detection provide a good separation efficiency and very high sensitivity for the analysis of such compounds. The detection limits for these species were about 75 micrograms/L when using conductivity detection and about 100 micrograms/L when using indirect UV detection. Because pH plays an important ...Continue Reading

References

Oct 21, 1983·Science·J W Jorgenson, K D Lukacs
Mar 1, 1994·Journal of Applied Toxicology : JAT·F R Sidell

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Citations

Dec 20, 2002·Journal of Chromatography. a·Edwin W J HooijschuurUdo A Th Brinkman
Nov 27, 1998·Journal of Chromatography. a·J P MercierA Tambuté
Aug 5, 2000·Journal of Chromatography. a·J E Melanson, C A Lucy
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Malin AllertHomme W Hellinga
Dec 28, 2006·Electrophoresis·Virginia Bernabé-ZafónGuillermo Ramis-Ramos
Apr 28, 2012·Electrophoresis·Acácio MendesAna Luísa Simplício
Feb 4, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Igor M Telles, Alexandre P Dos Santos
Nov 12, 2009·The Journal of Physical Chemistry. a·Li YangWilliam L Hase

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