Separation of cytokinin isomers with a partial filling-micellar electrokinetic chromatography-mass spectrometry approach

Electrophoresis
Liya GeEng Shi Ong

Abstract

A new method based on partial filling-MEKC (PF-MEKC) directly coupled to ESI-MS was developed for the simultaneous separation and determination of 13 structurally similar cytokinins, including various geometric and positional isomers of cytokinins. On the basis of the resolution of the neighboring isomer peaks, different parameters (i.e., pH and concentration of buffer, surfactant concentrations, length of the injected micellar plug, organic modifier, and applied separation voltage) were optimized to achieve a satisfactory PF-MEKC separation. Under optimum conditions, the separation of 13 cytokinin standards was accomplished within 25 min. MS/MS with multiple reaction monitoring detection was carried out to obtain sufficient selectivity. PF-MEKC-MS/MS allowed for the direct identification and confirmation of the cytokinins present in banana (Musa spp.) pulp sample after extraction and purification. Finally, trans-zeatin riboside (ZR) and trans-zeatin (Z) were unambiguously identified in banana pulp. It is anticipated that the current PF-MEKC-MS method can be applied to analyze cytokinins in a wide range of biological samples.

References

Jun 15, 1994·Biochemical and Biophysical Research Communications·S I Rattan, B F Clark
Sep 15, 1996·Analytical Chemistry·W M Nelson, C S Lee
Oct 23, 1997·Journal of Chromatography. a·L Yang, C S Lee
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·David WS Mok, Machteld C Mok

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Citations

Jan 5, 2012·Analytical and Bioanalytical Chemistry·Fuyou DuHuwei Liu
Feb 26, 2010·Journal of Chromatography. a·Govert W SomsenGerhardus J de Jong
Dec 8, 2011·Electrophoresis·Heide R RabanesJoselito P Quirino
Dec 21, 2010·Electrophoresis·Pavla PantůčkováLudmila Křivánková
Oct 8, 2016·Journal of Separation Science·Chaozhi PanLiya Ge

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