Oct 29, 1975

Separation of deoxyribonucleases (DNases) of normal human stratum corneum and psoriatic scales by micro-disc-electrophoresis

Archives of Dermatological Research
F J FörsterR K Zahn

Abstract

Normal stratum corneum and psoriatic scales were homogenized and a differential centrifugation was performed. The DNase activity of the individual fractions was investigated by micro-dis-electrophoresis. At pH 5 only in the 600xg pellet and 105.000xg supernatant of normal keratin DNase activity could be observed. However, all psoriatic fractions showed distinct enzyme activities. At pH 7.4 little psoriatic DNase activity could only be demonstrated in the 105.000xg supernatant. Except from the 15.000xg pellet all fractions of normal stratum corneum displayed marked activities. In addition the 105.000xg supernatant showed two different DNase bands.

  • References3
  • Citations6

References

Mentioned in this Paper

Psoriasis
Deoxyribonuclease I
Pellet Formation
SDS-PAGE
Keratin
Cytokeratin
Centrifugation
Alkaline DNase
Supernatant
Deoxyribonuclease I Activity

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