PMID: 974Oct 29, 1975

Separation of deoxyribonucleases (DNases) of normal human stratum corneum and psoriatic scales by micro-disc-electrophoresis

Archives of Dermatological Research
F J FörsterR K Zahn

Abstract

Normal stratum corneum and psoriatic scales were homogenized and a differential centrifugation was performed. The DNase activity of the individual fractions was investigated by micro-dis-electrophoresis. At pH 5 only in the 600xg pellet and 105.000xg supernatant of normal keratin DNase activity could be observed. However, all psoriatic fractions showed distinct enzyme activities. At pH 7.4 little psoriatic DNase activity could only be demonstrated in the 105.000xg supernatant. Except from the 15.000xg pellet all fractions of normal stratum corneum displayed marked activities. In addition the 105.000xg supernatant showed two different DNase bands.

References

Apr 1, 1972·The Biochemical Journal·A J Barrett, J T Dingle
May 1, 1972·Laboratory Animals·A I Walker, J W Barrett

Citations

Dec 27, 1977·Archives of Dermatological Research·G LeonhardiA Neufahrt
Oct 27, 1976·Archives of Dermatological Research·G TeimerG Leonhardi
Sep 27, 2003·Methods : a Companion to Methods in Enzymology·Kristina K PeachmanC R Alving
Mar 11, 2011·PloS One·H FischerL Eckhart
Mar 1, 1981·The Histochemical Journal·G Huether, V Neuhoff
Jan 1, 1983·Archives of Dermatological Research·R OguraR K Zahn

Related Concepts

Alkaline DNase
SDS-PAGE
Hydrogen-Ion Concentration
Alloenzymes
Psoriasis
Skin
Subcellular Fractions

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