PMID: 3766959Aug 15, 1986Paper

Separation of dimethylphosphatidates of alkylacyl glycerophosphocholine and their molecular species by high-performance liquid chromatography

Analytical Biochemistry
Y NakagawaK Waku

Abstract

A high-performance liquid chromatography (HPLC) method for separation of the alkylacyl and diacyl analogs of choline glycerophospholipids (CGP) of guinea pig polymorphonuclear leukocytes and their molecular species is described. CGP were hydrolyzed with phospholipase D and then methylated with diazomethane to convert them to dimethylphosphatidates. The dimethylphosphatidates were then separated into the alkylacyl and diacyl subclasses by HPLC on a silica gel column within 15 min. The alkylacyl and diacyl analogs were then separated into individual molecular species by reverse-phase HPLC. Dimethylphosphatidates were resolved into 15 separate peaks, and 11-16 different molecular species of alkylacyl and diacyl glycerophosphocholines were identified on gas-liquid chromatography. The present results indicate that the CGP of polymorphonuclear leukocytes are composed of 27 major molecular species. In the alkylacyl subclass, the most predominant species was the 16:0-18:2 species (32%), followed by the 18:1-18:2 (18%), 16:0-16:0 (16%), and 16:0-18:1 (15%) species. The diacyl type consisted mainly of species with 18:2 at the 2-position, such as the 16:0-18:2, 18:0-18:2, and 18:1-18:2 species, the total percentage of which was 57%.

References

Mar 16, 1971·Biochimica Et Biophysica Acta·A MontfoortL L van Deenen
Sep 1, 1972·The American Journal of Physiology·R J King, J A Clements
Jan 1, 1981·Annals of the New York Academy of Sciences·B B VargaftigF Wal
Oct 14, 1982·Biochimica Et Biophysica Acta·V G Mahadevappa, B J Holub

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