PMID: 11318418Apr 25, 2001Paper

Separation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: potential for emergency diagnosis of inborn errors of metabolism

Journal of Chromatography. B, Biomedical Sciences and Applications
O BoulatD D Chen

Abstract

Free amino acids (AAs) in human plasma are derivatized with 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) and analyzed by capillary electrophoresis (CE) with laser induced fluorescence (LIF) detection. The labeling procedure is significantly improved over results reported previously. Derivatization can be completed in 40 min, with concentrations as low as 4 x 10(-8) M successfully labeled in favourable cases. Twenty-nine AAs (including 2 internal standards) are identified and can be reproducibly separated in 70 min. Migration time RSD values for 23 of these AAs were calculated and found in the range from 0.5 to 4%. The rapid derivatization procedure and the resolution obtained in the separation are sufficient for a semi-quantitative, emergency diagnosis of several inborn errors of metabolism (IEM). Amino acid profiles for both normal donor plasma samples and plasma samples of patients suffering from phenylketonuria, tyrosinemia, maple syrup urinary disease, hyperornithinemia, and citrullinemia are studied.

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Citations

Feb 5, 2002·Electrophoresis·C PrataF Couderc
May 15, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun-Bo ZhangJian-Nong Ye
Jul 10, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kevin H Carpenter, Veronica Wiley
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gordana ZunićSlavica Spasić
Jul 26, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Georg Hempel
Jan 24, 2004·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Gordana Zunić
Jun 18, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ji-Seon JeongSeon-Pyo Hong
Mar 26, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Worapan PormsilaPeter C Hauser
Mar 15, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Naz ShamaByung Hwa Jung
Dec 12, 2007·Electrophoresis·Elaine Teng Teng TaySam Fong Yau Li
Jan 18, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Petr TůmaKaterina Andelová
Jun 5, 2019·Electrophoresis·Lucie PerquisFrançois Couderc
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc

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