Separation of geometric isomers of native lutein diesters in marigold (Tagetes erecta L.) by high-performance liquid chromatography-mass spectrometry

Journal of Chromatography. a
Rong TsaoTony Manolis

Abstract

Lutein is found in many foods; the richest and purest plant source is marigold flower (Tagetes erecta L.). In this plant, lutein is in the form of saturated fatty acid diesters. By using a binary mobile phase consisting of ethyl acetate and acetonitrile-methanol (9:1), improved separation was achieved on a C18-bonded phase. The unique absorption of lutein cis isomers at 330nm was used in combination with MS to identify the novel cis-lutein isomeric dimyristate, myristate-palmitate, dipalmitate, and palmitate-stearate diesters, as well as the rare combinations of both trans- and cis-lutein laurate-palmitate and trans- and cis-lutein myristate-stearate. The presence of the all-trans-lutein laurate-myristate, dimyristate, myristate-palmitate, palmitate-stearate, and distearate diesters, reported by others, was also confirmed.

References

Nov 24, 1999·Journal of Agricultural and Food Chemistry·F KhachikH Pfander
Feb 20, 2003·Journal of Agricultural and Food Chemistry·Julia M Humphries, Frederick Khachik

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Citations

Jul 8, 2005·Applied Microbiology and Biotechnology·Prakash Bhosale, Paul S Bernstein
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rong Tsao, Zeyuan Deng
Jul 17, 2014·Biological Research·Porkaew PromphetDuangkamol Kunthalert
Oct 27, 2015·Journal of Agricultural and Food Chemistry·El-Sayed M Abdel-Aal, Iwona Rabalski
Jun 3, 2005·Phytochemical Analysis : PCA
Apr 14, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Snezana Agatonovic-KustrinDavid W Morton
Jun 1, 2007·Journal of Agricultural and Food Chemistry·J Christopher YoungBarbara A Blackwell
May 7, 2010·Journal of Agricultural and Food Chemistry·María del Carmen Cerón-GarcíaEmilio Molina-Grima
Aug 26, 2010·Journal of Agricultural and Food Chemistry·El-Sayed M Abdel-AalIwona Rabalski
Sep 27, 2007·Journal of Agricultural and Food Chemistry·Wei LiQi Wang
Feb 1, 2007·Journal of Agricultural and Food Chemistry·El-Sayed M Abdel-AalJudith Fregeau-Reid

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