Separation of highly fluorescent proteins by SDS-PAGE in Acroporidae corals

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M PapinaH Yamasaki

Abstract

This study characterized the spectral properties of Acropora tenuis, A. nasuta, A. secale, and A. aspera, all of which showed strong colorful fluorescence under ultraviolet light-A (black light). The emission maxima of fluorescence from the intact corals were 517, 482, 484, and 514 nm in A. tenuis, A. nasuta, A. secale, and A. aspera, respectively. Using a soluble fraction of cell-free extract of the corals, we applied a method of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to separate each fluorescent protein component contained in the corals. Green fluorescent bands were detected in all Acropora examined, although their apparent molecular mass and relative content were different. A. aspera had two orange bands in addition to the green one. The major excitation and emission peaks of the orange fluorescence bands were almost identical (476 and 478 nm), however, they were discernible by their spectral profiles and molecular masses. Some biochemical properties of the highly fluorescent proteins of Acropora are described and implications of the results are discussed.

References

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Citations

Apr 8, 2006·Plant Methods·Alexander SchulteChristoph Plieth
Dec 21, 2010·PloS One·Stanley S C Wong, Kevin Truong
May 27, 2016·The Journal of Biological Chemistry·Mona LindePatrick Babinger

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