Separation of human cells bearing HLA-DR antigens using a monoclonal antibody rosetting method

Tissue Antigens
J W StockerR Ceppellini

Abstract

A technique is described for enriching, from human blood, cells bearing HLA-DR antigens. The method depends on the use of monoclonal mouse antibody which reacts with HLA-DR structures. Cells to which this antibody has bound can be separated after rosetting with bovine erythrocytes coated with anti-mouse immunoglobulin. The cells thus enriched may be used for HLA-DR typing by standard cytotoxicity methods with allogeneic sera.

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Citations

Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·F G GudatJ P Obrecht
Jun 1, 1981·Journal of Cutaneous Pathology·E M McMillanM A Everett
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Jan 1, 1981·Journal of Immunological Methods·J S NowakD G Gilmour
Feb 1, 1981·Analytical Biochemistry·G S Eisenbarth

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